We narrowed to 191 results for: pme i

...And while I have it on good authority that a complete Western blot can be run in a single day, I really ... while providing needed flexibility in assay development. Researchers who understand how and why to use...

...pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP...specific cell types are missing in roots. The development of transgenic hairy roots takes at least six ...scientists do transient leaf transformations The development of transgenic plants is a common problem in non-model...might provide some useful guidelines for the development of transient transformation systems for other...

...Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019... on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer...

...species, with components quickly losing activity. I set out to collect the features that would define ...effectors from nanobodies to fluorescent proteins. So, I hope you will take full advantage and have lots of...is made possible through the utilization and development of genetically engineered components, like protein...

...find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest...electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful if you...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from...

... work for your application? Like many scientists, I have spent hours sifting through comments and papers...is a public service to the science community, and I am excited to be a part of it! If you are interested... a wrong section. The antibody screening and development of a focused reference bring us one step closer...

... vector that encodes both Cas9 and sgRNA. What if I want to work with gene drives? Then do so! It's a ...Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound...contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical...

...101: An Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & ...., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M., & Gradinaru, V. (2022). Engineered AAVs for non-invasive....ymthe.2019.02.013 Huang, Q., Chan, K. Y., Tobey, I. G., Chan, Y. A., Poterba, T., Boutros, C. L., Balazs...Azari, B., Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., ..., J., Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin...Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G.... L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M., & Sabeti, P. C. (2023). Systemic...

... be flat out better than SpCas9. I want everyone to test it because I want to know the answer", says Liu...changes compared to SpCas9 in parentheses. The development of xCas9 3.6 and 3.7 shows that we have only ...

...-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on the neurobiology...opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote...high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human...enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3 (2012): 404-414. PubMed PMID...

...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow multicolor...Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260-6. doi: 10.1038... were very time-intensive, necessitating the development of a new technique. In a structure as tightly...lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium. For scientists interested...interested in neuroscience and development, Brainbow is a valuable tool to mark and follow single cells... Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631...

...should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...can be used to overcome this (8). The recent development of tissue clearing techniques (9) now allows ...

...journal.pgen.1010765.  Berlew, E. E., Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C., Jaber, Z., Gardner,....7554/eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. ...critical when, say, the gene is essential during development and a knockout is not feasible or when the protein...Rab proteins during a key stage of zebrafish development (Aljiboury et al., 2023). Rab family proteins...formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https://doi....

... the concentration of the unknown sample.  How do I make the standards for a standard curve? You create...should be within 15% of the expected value. How do I plot and use a standard curve? To create a standard...depending on a number of factors, including the user, equipment, assay incubation time, etc., which can vary even...

...the American Type Culture Collection aka ATCC where I work. It is virtually impossible to work in cell culture...virology without acquiring materials from this bank. I am often asked, “What is the difference between materials...backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial can then be thawed...

...Gaudelli et al., 2017 Adenine base editors (ABE7.10)   A→I (A→G) editing with high product purity and low off-target..., H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base editing of...Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020...elements were the starting point towards the development of the first cytosine base editors from Alexis... base editing scope and efficiency Since the development of BE3, many research groups have made improvements...guanine (Thuronyi et al., 2019). Following the development of adenine base editors (discussed next!), RNA...constrained by PAM availability in the genome. So the development of near-PAMless Cas9 variants significantly expands...

... Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about...blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here are Five...

..., Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the last few years...

...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...entirely genetically encoded do not exist, but the development of non-natural fluorescent amino acids and their...

..., H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base editing of...Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020...such a modular platform could accelerate the development of genetic therapies even further. In the meantime...