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We narrowed to 188 results for: pme i

Showing: 121 - 140 of 188 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...And while I have it on good authority that a complete Western blot can be run in a single day, I really ... while providing needed flexibility in assay development. Researchers who understand how and why to use...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP...specific cell types are missing in roots. The development of transgenic hairy roots takes at least six ...scientists do transient leaf transformations The development of transgenic plants is a common problem in non-model...might provide some useful guidelines for the development of transient transformation systems for other...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019... on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer...

  4. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ... work for your application? Like many scientists, I have spent hours sifting through comments and papers...is a public service to the science community, and I am excited to be a part of it! If you are interested... a wrong section. The antibody screening and development of a focused reference bring us one step closer...

  5. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest...electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful if you...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from...

  6. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... vector that encodes both Cas9 and sgRNA. What if I want to work with gene drives? Then do so! It's a ...Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound...contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...101: An Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & ...., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M., & Gradinaru, V. (2022). Engineered AAVs for non-invasive....ymthe.2019.02.013 Huang, Q., Chan, K. Y., Tobey, I. G., Chan, Y. A., Poterba, T., Boutros, C. L., Balazs...Azari, B., Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., ..., J., Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin...Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G.... L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M., & Sabeti, P. C. (2023). Systemic...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... be flat out better than SpCas9. I want everyone to test it because I want to know the answer", says Liu...changes compared to SpCas9 in parentheses. The development of xCas9 3.6 and 3.7 shows that we have only ...

  9. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on the neurobiology...opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote...high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human...enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3 (2012): 404-414. PubMed PMID...

  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow multicolor...Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260-6. doi: 10.1038... were very time-intensive, necessitating the development of a new technique. In a structure as tightly...lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium. For scientists interested...interested in neuroscience and development, Brainbow is a valuable tool to mark and follow single cells... Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...can be used to overcome this (8). The recent development of tissue clearing techniques (9) now allows ...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...journal.pgen.1010765.  Berlew, E. E., Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C., Jaber, Z., Gardner,....7554/eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. ...critical when, say, the gene is essential during development and a knockout is not feasible or when the protein...Rab proteins during a key stage of zebrafish development (Aljiboury et al., 2023). Rab family proteins...formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https://doi....

  13. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... the concentration of the unknown sample.  How do I make the standards for a standard curve? You create...should be within 15% of the expected value. How do I plot and use a standard curve? To create a standard...depending on a number of factors, including the user, equipment, assay incubation time, etc., which can vary even...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...the American Type Culture Collection aka ATCC where I work. It is virtually impossible to work in cell culture...virology without acquiring materials from this bank. I am often asked, “What is the difference between materials...backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial can then be thawed...

  15. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Gaudelli et al., 2017 Adenine base editors (ABE7.10)   A→I (A→G) editing with high product purity and low off-target..., H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base editing of...Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020...elements were the starting point towards the development of the first cytosine base editors from Alexis... base editing scope and efficiency Since the development of BE3, many research groups have made improvements...guanine (Thuronyi et al., 2019). Following the development of adenine base editors (discussed next!), RNA...constrained by PAM availability in the genome. So the development of near-PAMless Cas9 variants significantly expands...

  16. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about...blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here are Five...

  17. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ..., Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018...-2 RNA, but inadequate access to reagents and equipment has become a bottleneck. In the last few years...

  18. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...entirely genetically encoded do not exist, but the development of non-natural fluorescent amino acids and their...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am I ready for CRISPR? A user’s guide to genetic screens...s41587-020-0490-7 Radzisheuskaya A, Shlyueva D, Müller I, Helin K (2016) Optimizing sgRNA position markedly...is still the most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered...
Showing: 121 - 140 of 188 results