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We narrowed to 286 results for: prep

Showing: 121 - 140 of 286 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Resources on Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...intracellular transport machinery. Addgene offers viral preparations of AAV2-retro vectors with a variety of transgene...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share ...alongside a journal article, a preprint, or without any paper at all. Posting preprints and openly sharing materials...reproducibility, and accelerate science. We encourage preprint authors to deposit materials without a hold or...or to release the hold as soon as the preprint is posted in order to support these goals. And we can update...article version any time later. We recognize that a preprint isn’t necessarily a clear sign that you are ready...your plasmids, so we won’t immediately release preprint-associated materials the way we will with journal...to consider sharing them openly as soon as that preprint is online!   Thanks for reading, and for depositing...

  3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...confirm the identity and serotype of our AAV preparations. We recently published a description of the ... For labs that produce a small number of AAV preparations this level of quality control is sufficient ...facilities producing a large number of viral preparations in parallel, there is a very real risk of mixing...AAVs and the TA-based ligation and tagmentation preparation approaches described previously require a preliminary...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...simplified approach, we sequenced over 100 AAV vector preparations and showed that VGS can quickly and reliably... script that can confirm the serotype of AAV preparations.  Using viral genome sequencing to determine...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...first scientific poster, but unsure how best to prepare. Maybe you’re a presentation veteran, but have ...experience. No matter the reason for attending, your preparation and day-of game plan will ensure you have the...experience possible. Tips for poster session presenters Prepare for success You’ve put a great deal of thought ...it’s time to expertly show off your science.  Prepare the night before: To avoid rushing out the door...poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our posters... part of a poster session, but with a little preparation and a few deep breaths the day-of, you’ll handle...Strategically traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ...for poor quality slides with sufficient time to prepare (and it does take time). For important talks, ask...loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few extra slides...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...their phones. Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than...

  6. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...consider using a different antibody.  Prepare a positive control Prepare and label a sample that expresses...paraformaldehyde-fixed cell samples, or other preparations. Sequential IF For a sequential IF experiment... ICC validated antibodies from Addgene), or be prepared to run some extra controls and validate the antibody...expect the target to be. You can test this by preparing a sample without the primary antibody, or with...different antibodies, blocking, washing, and sample preparation steps, in order to ensure your antibodies are...

  7. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ... Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...Research to make competent cells because cells prepared using this kit can be transformed without heat...of electrocompetent cells (protocol)  Cells prepared using the Z-competent TM buffer can be easily ...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...containing instructions on how to prepare your plasmids and a prepaid label with shipping materials to ...journal. To start this process, simply enter your preprint title or a general description of the deposit....

  9. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ... can prepare you to present your work at conferences, help with manuscript and grant preparation, and ...science communication skills. Ultimately, my degree prepared me to be exactly the type of scientist I wanted...interview with them to learn how their master's degree prepared them for their career.  2. Are you looking for...

  10. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...while meditating right before bed will help you prepare for sleep. At Addgene, our mindfulness group is...more about career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids...tutorials for all ages. Organize your data and prepare publications or your dissertation Organize data...write introductions to potential publications or prepare a review article. These are both options that can...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing replication factors and serotype-specific...been developed to determine the titers of rAAV preparations, such as dot blot, enzyme-linked immunosorbent...overestimate of transducible units in the virus preparation.  rAAVs  Now your AAV virions are ready to store...Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580...

  12. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each... could affect the measurements. You'll need to prepare the standard, standard dilutions, and unknown sample...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...every time the assay is run, ideally with freshly prepared buffer. This is because standard curves vary depending...

  13. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...personality types, and to devise strategies to be prepared for addressing unexpected situations. At the end...long-term? A few years as a healthcare consultant can prepare you for a successful career in several different...foot in the door When I was first considering preparing for and transitioning to a career in healthcare...learning about the nature of the job and the ways to prepare for it. Besides those conversations, reading online...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...should be consistent over time and from different preparations of the purified test plasmid expressing the ... with purified plasmid from the same aliquot/preparation. The replicate control isn’t measuring or assessing...Using our example above, we would test different preparations of Plasmid A in different aliquots of cells ...singular or associated with only one aliquot/preparation of the test plasmid.  Let us now revisit our ...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ... can then be purified for subsequent library preparation and high-throughput sequencing. Improvements ...lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the initial CUT... in terms of initial steps of cell or tissue preparation.   All in all, CUT&RUN offers a great alternative...

  16. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...buy a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert ... (in the U.S.). Their cooler boxes come with a prepaid return shipping label. To return the box, simply...

  17. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

  18. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...Here are a few things to consider when you're preparing your resume for a non-academic position: What ...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,..., but the earlier you ask yourself, the better prepared you are for your next step. Here are some great...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...persuading an employer that your past work has prepared you to contribute at a high level to their team...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...form? Training in science communication should prepare you to ask and answer these questions for a diverse...
Showing: 121 - 140 of 286 results