We narrowed to 183 results for: rato cre

...amazing years in front of us. So in our last celebratory moment, we wanted to walk through some of our...it, it just reflects how useful the reagents we created are for the community. More than 100 labs worldwide...need for high-quality service, open data, and increased reproducibility of materials. In March 2022, we... supported by an NIH BRAIN Initiative. We also created a growing library of antibody educational resources...York, Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists who have joined...

...early career scientists taking an active part in creating their own opportunities for career development...in the Netherlands. There are many academic administrators who are finding ways to provide new types of...

...Factors to consider when creating a pooled library amplification protocol. Created with BioRender.com. ...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Re-transformation of the SPLINTR Libraries (PDF, 401 KB) Screening Zhang Lab - Multiplexed Overexpression of Regulatory...et al. Nat Protoc. 2017 with modifications Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis...Amplification and Sequencing Protocol (DOCX, 32 KB) Screening Rinehart Lab Iterative Synthetically Phosphorylated...-Cas9 knockout and transcriptional activation screening, a protocol paper by the Zhang lab, and the Broad...More resources on the Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality...

...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...samples from several plates are pooled together to create a single library containing fragments carrying ...

...relevant science."     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking...

...encoding Gag and Pol, increasing the number of recombination events necessary to create a replication-competent...Enable Genome-Scale, Knockout Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...shows how the lentiviral genome was condensed to create the 2nd-generation lentiviral system (Figure 2)...safer than the original system, the possibility of creating a replication-competent virus via recombination...possibility and enhance biosafety, Dull et al. created the 3rd generation system (Figure 3), which differs...Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab - the Trono lab’s lentiviral...

..., social media, coffee chats with potential collaborators, blogging and more. These each have different...publishing has a number of drawbacks, especially the increasingly growing time from submission to publication....for their efforts, inspire future research, and increase their impact. Open access and transparency Another...journal publication or an updated preprint, further increasing their findability. Now, go out there and finish...

... aaRSs in these processes. To create the antibody toolbox, they screened antibody candidates using a synthetic...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...fourteen aminoacyl tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins...

...compose the secretory pathway, endocytic vesicles, mitochondria, lysosomes or they are secreted into the ...your cloning strategy and decide whether you will create an N- or C-terminal fusion based on your experimental...residues form inappropriate disulfide bonds in the secretory pathway (Figure 1) [4,6–8]. Additionally, FPs ...and a sequence intended to localize them to the secretory pathway, a dark pool that is mislocalized to the...misfolded, blocked from proceeding through the secretory pathway, and retained in the ER. FPs have been...recent publication illustrated the quantitative increase in fluorescent signal achieved when utilizing ...optimized moxFP as opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather...

...ve learned that increased accessibility is achieved through interconnected incremental changes. It was...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...identified and offered a number of solutions that increased accessibility, such as using couriers that refill...In some cases, the distributors broadly helped increase access in the country, and we can see a growth...re continuing to use our data and resources to increase accessibility to researchers around the globe....the materials in our repository. We started to increase accessibility to our repository by making it easier...Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid...

... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio...

...Hayward et al., 2024). The fluorescence intensity decreased whenever the magnet approached the sample (by ... and the team set up a directed evolution and screening experiment with three of the proteins that had...several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no... suggest she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

...demonstrated to result in decreased mismatch tolerance and, consequently, increased specificity relative to...more stringent targeting rules that result in a decrease in available target sites. Regulating gene expression...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... scientific discovery through the creation of innovative technologies. To date his group has develop several...

...White House as a Champion of Change, a program created as part of President Barack Obama's Winning the...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...DNA. We thought these were so much fun and so incredibly useful that we wanted to make them free to use... the invention of genetic engineering. What's incredible is the bioeconomy is already pretty substantial...product – equal to mining. There is an absolutely incredible hidden story here. The parts we are releasing...

...list of biosensors too, as another laboratory may have already created the sensor that you need. FRET biosensors...available on our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids...multiple FRET biosensors simultaneously would further increase the amount of correlated information available...

...Figure 1: Common functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...years, and new classifications are still being created as whole-genome sequencing becomes more relevant...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

...much information as possible from depositing laboratories and associated publications, but our quality... we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection and...bioinformatics workflow management system As novices to creating a robust data pipeline that would be reliable ...community of Nextflow supporters existed who had created a shared resource of pipelines for genomic data... with others without any complicated setup. We created, tested, and debugged each of our pipeline steps...running the pipeline locally and in the cloud. Creating a bioinformatics workflow in Nextflow Establishing...scripting language that can be executed on Linux, we created our first process by simply copying and pasting...

...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...incubation time to maintain cell viability and increase protein production (Schwarz et. al., 2020). Harvest...   Figure 1: The steps of rAb production. Created with biorender.com   Another method of harvesting...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...contaminating bands or a few strong ones, consider increasing the number of washes performed during your harvest...

... I’ll describe an episomal vector system that increases reprogramming efficiency through the expression...successfully to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently...requires stringent biosafety measures, thereby increasing the costs of the procedure. Episomal reprogramming...reprogramming is an ideal and cost-effective method for creating clinical-grade, safe, nonviral and nonintegrating...episomal reprogramming cocktail by Okita et al. increased the reprogramming efficiency in PBMCs [7]. The...transiently. The transiently expressed EBNA-1 increases protein expression from the other episomal plasmids...used on PBMCs, the pCXLE episomal vector system increases the efficiency of reprogramming from 0.001% to...

...humanities, and 32% in the social sciences go uncited. Creative titles and controversial topics can garner some...Article, since the 1980s researchers have been increasingly citing research from outside of their disciplines...interdisciplinary research while at the same time increasing interdisciplinary citations in their own work...instant sharing across multiple other sites), I created a new platform for sharing video abstracts about...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...effectively.” How to share your science through video Creating an effective video about your research is not ...offers a 5-step process and several examples for creating effective research videos.  Karen McKee, also...