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...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

...comments below. And if you want to give GeoCas9 a try, as always, the plasmids are available from Addgene...

...campsite. And it was exciting – one night while trying to process samples at the picnic table before dark...

... “pending.” Fear not, there are many ways we’re trying to make MTA turnaround faster with technology transfer...

... the protein as it exists in your assay?  First, try to understand, to the best of your ability, what ...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

...actively testing it in other applications! If you try it out in a new assay, let us know. We’d love to ...

...failing, over and over again until you don't, of trying to understand your completely unexpected findings...

...particular sections. The focus groups allowed us to try out techniques that were discussed in the larger ...

...us acquire a lot of those plasmids so that we can try many different things in parallel.” Alternating Hemiplegia...

...about their work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission...

...entire series as it is published. For more reading try The Discipline of Teams by Jon R. Katzenbach and ...

...progress. To illustrate this point, consider that I try to exercise every morning, but the temptation to ...

...bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant excitation/emission...bleeding, or spectral overlap, and why you should try to avoid or reduce it whenever possible. Finally,...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Flow Compensation...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...reporters, and/or dye stains used in your flow cytometry experiment are known as your "panel". In this ...from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation...degree. The phenomenon of spectral overlap in flow cytometry is commonly referred to as bleeding. When two ...

...have mastered this technique so it might be worth a try, especially since the potential rewards could be ...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko...distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening...23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as a spectroscopic...spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID: 354506. 12. Vilensky...

...lectures should be “brains-on”, but as much as I try being compelling, interactive, and application-driven...

...comparisons.  We very much appreciate feedback, so try out the site and contact us if you have any ideas...

... we are all so good with video calls, how about trying your hand at a video presentation? Most video platforms...

...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...

...any labs performing genome editing experiments to try nucleofection as (in our hands) this technique supports...

... on this topic and for additional best practices try "Why Aren't You Delegating" in the Harvard Business...