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We narrowed to 464 results for: try

Showing: 121 - 140 of 464 results

  1. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...fewer and fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge...plastic materials are fairly inexpensive in this country and we are quick to throw out chipped or imperfect...

  3. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...comments below. And if you want to give GeoCas9 a try, as always, the plasmids are available from Addgene...

  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ... the protein as it exists in your assay?  First, try to understand, to the best of your ability, what ...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...actively testing it in other applications! If you try it out in a new assay, let us know. We’d love to ...

  7. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...failing, over and over again until you don't, of trying to understand your completely unexpected findings...

  9. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...particular sections. The focus groups allowed us to try out techniques that were discussed in the larger ...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant excitation/emission...bleeding, or spectral overlap, and why you should try to avoid or reduce it whenever possible. Finally,...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Flow Compensation...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...reporters, and/or dye stains used in your flow cytometry experiment are known as your "panel". In this ...from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation...degree. The phenomenon of spectral overlap in flow cytometry is commonly referred to as bleeding. When two ...

  15. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...have mastered this technique so it might be worth a try, especially since the potential rewards could be ...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko...distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening...23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as a spectroscopic...spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID: 354506. 12. Vilensky...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...
Showing: 121 - 140 of 464 results