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We narrowed to 456 results for: val

Showing: 121 - 140 of 456 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...from that country.   While written resources are valuable, there does come a time when a picture is worth... favorite measure of our educational resources’ value is feedback from the researchers who use them. The...website survey on addgene.org. Currently, we’re evaluating our depositor process to identify and reduce ...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their accuracy, specificity...the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to ...

  3. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a pooled library...complexity of pooled libraries, NGS is the best way to validate your amplified library and verify that you have...lentiviral production, transduction, screening, and validation of CRISPR libraries. The Genome Engineering using...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... foster a culture of inclusivity. Work with and value people who think differently from you and you will... share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New...come up with any ideas obviously worth pursuing, evaluate the “bad” ones. Bad ideas are easier to work with...

  5. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus...will fold and that the fluorescence pattern is equivalent to the distribution of FP-fusion proteins. Our.... Pubmed Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags...

  6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ... https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer SJ, Luna-Velez MV, Xiao...Xiao R, Bennett J, Vandenberghe LH (2017) Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting.../mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...or disagreeable, you recognize their feelings as valid. Listening: In one of my favorite books, Michael... means the other party will feel punished or invalidated. Both compromise and arbitration might remedy...bringing this asset to your job, you can become a valuable asset yourself.     Fig 2: A diagram of...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...anticipated restriction analysis results (left) and validation PCR followed by Restriction Fragment Length Polymorphism...introduced restriction site is also duplicated. Validating your mutagenized plasmids Once constructs containing...confirmed to be free of primer duplications), final validation is performed by sequencing functional regions...

  9. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...spectrometry suggest that phosphorylation is far more prevalent than previously appreciated. PhosphoSitePlus, ...as described below) provides researchers with a valuable tool for understanding the contribution of phosphorylation...glutamate which allows the system of interest to be validated first through the production of full-length protein...

  10. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... about you. It’s worth taking time to honestly evaluate your own style of work and communication before...acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders of blunders...lab for you is out there if you take the time to evaluate the options critically. How will it feel when ...

  11. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...human extracellular proteins with community-based validation. They also have plans to expand to other protein... NEB and ATCC can provide you with additional validated reagents, and, with these reagents in hand you... of causation from initial hypothesis through validation are easier to understand. The need for great ...

  12. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...tumour suppressor. This summer role was incredibly valuable because it was a set duration of only three months...previous experience working in Industry was really valuable; I had received all the training and experience... required a scientist for a 6 month project to validate some genomic changes, so this arrangement was ...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...steps? Do you know how the reagents have been validated? How will the results fit into a future manuscript...complex, here are a few guidelines to help you evaluate projects:   If all of your experiments work as...engaging talk takes significant effort. It requires evaluation of all of the data and any recent relevant studies...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...who decides how they are going pursue regulatory approval for a new product or a well-endowed philanthropy... position and will likely be the bulk of your evaluation criteria in interviews or essays. Note: the answer...societies, industry, and beyond!  Learn and develop valuable science policy skills in graduate school via this...

  15. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...choosing the right lab. Remember that your time is valuable! Don’t jump on the first opportunity that comes...when I was in high school, but I’ve learned the value in finding mentors wherever I go. This story by ...found that the advice in these resources is still valuable for anyone in the lab though, including undergrads...

  16. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...change the fly lab experience. The Frequency Light Interval System, or FLIS, incorporates a bioluminescence...of light, visible to the naked eye, at regular intervals. This innovation system can help fly labs everywhere...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... had more robust initiation of reprogramming (evaluated by an alkaline phosphatase-positive assay) as ...across species and will undoubtedly constitute a valuable tool for the research community. You can find ... and were carefully designed to allow for easy removal of the resistance cassette via Cre recombination...

  18. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Antibodies, who shared their antibody validation expertise. Antibody Validation for Flow Cytometry Rob Hurt,...

  20. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ... interest to the depositor, but it can also be valuable data to share in grant proposals or to demonstrate...through, aggregation of this information can provide valuable insight to the most requested plasmid, the number...
Showing: 121 - 140 of 456 results