We narrowed to 884 results for: ica

...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal... Jesse Zalatan’s lab deposited plasmids for a chemically inducible CRISPRa system in yeast is mediated... channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use...

...can contain different neurotransmitters. Using genetically encoded fluorescent proteins coupled with Fibronectin... show a high proportion of blue fluorescence, indicating cell proliferation. BaF3 cells maintained in ...in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle. Cells that had IL-3 withdrawn...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

...methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al....themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

... first at Brigham and Women’s Hospital/Harvard Medical School and then Boston University (institutions...committees would not function without long distance communication and learning to work with people despite the...the distance improved my verbal and written communication skills. Volunteering on these committees was ...Chair then stepped down, I became interim Chair automatically as it was an understood expectation of any Vice-Chair...

...detect explosives and rapidly indicate their presence. They are genetically modified organisms, and GMOs...things more interesting, we are working in a new technical discipline that nobody understands (synthetic ...that survived the literature review, survived technical feasibility analysis, and was a marketable product...

...genome editing technology that allows precise modifications of DNA (replacements, small insertions, and ... Liu, 2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase... design of the pegRNA has been shown to play a critical role in the editing efficiency of prime editing...configurations can be used to introduce specific target modifications. Since this means we could design hundreds to...

...setup. For instance, BV510 and AmCyan have almost identical emission spectra but may be called BV510 on one...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...shift towards the X axis to become a perpendicular vertical line.     In general, most spillover between samples...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

...your gene of interest is difficult to target specifically with a gRNA). Be sure to keep these techniques...sequences are exchanged between two similar or identical molecules of DNA. Gene targeting takes advantage...homology is required, but 6 to 14 kb of homology is typical for targeting constructs. In the example shown ...cells participating in development or other physiopathological events to which the chosen promoter responds...

...These impurities cannot be removed during the purification step because they are inside the virus itself...sequence (gene). In a clean sample, there are typically fewer than 10 hits to a given sequence, and we...Guerin et al., 2020).  In addition to genome identification, we developed custom open-access Python scripts...control is recommended but not yet required for clinical-grade materials, and is not commonly performed...

...to the same principles listed here. It employs biological best practices. For example, it ranks guides ...with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello ...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...published on how to do it best. Scientists are typically advised to mutate the PAM to which the guide binds...