...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...

...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...

... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...

...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna...

... the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...directed evolution, because channelrhodopsins are typically evaluated with low-throughput methods such as ... midbrain, fiber-optic cannulas were still stereotaxically implanted into the mice’s brains so that light...

... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...to your values While going through the co-op application process, my advisor encouraged me to keep my ...

...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...

...s outer membrane and allows the components to physically enter the cells so they can go to work. ...Allen Cell Explorer, where you can also find the certificate of analysis provided with each cell line. The...