We narrowed to 884 results for: ica

...incredibly useful, yet simple system allows you to systematically study gene function with temporal control and...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the coordinators...

...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols...

...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...PI labs and institutions. There are many more technical details that make our new search engine unique...

...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...CRISPR-Cas9 to increase its specificity." Nature communications 9.1 (2018): 3048. PubMed PMID: 30082838. PubMed...CRISPR-Cas12b for human genome editing." Nature communications 10.1 (2019): 212. PubMed PMID: 30670702. PubMed...

...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...

...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...

... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...

...other proteins that require post-translational modification may not be easily expressed in non-mammalian...are intended for the high level expression and purification of SARS-CoV-2 proteins for downstream experiments...and we’re glad to be able to do so during this critical time. Visit our COVID-19 Plasmids and Resources...