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We narrowed to 884 results for: ica

Showing: 761 - 770 of 884 results
  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...Anything between orange and light orange usually indicates that the cells have grown enough, are making their...analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ...
  2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...directions. Drosophila are very easy to manipulate genetically and have limited genetic redundancy (meaning,...reference of various fly phenotypes, check out the Classical Genetics Simulator website. One interesting thing... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...
  3. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...their fused protein under certain conditions (typically the addition of an exogenous small molecule). ...in size from several kDa up to ~35 kDa and are typically knocked-in via gene targeting or cloned in-frame... F-box protein must be expressed (inducibly, ectopically, stably… it’s your call). The TIR1 F-box protein...
  4. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...:  my undergrads were different people! More specifically, they had different learning styles. One was...delegating well, but it does get easier over time! Communicate often and clearly As a manager you need to clearly...it is the best way to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable...
  5. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...
  6. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... to synthesize a large volume of information: basically, a scientist’s tool kit. I wrote a review article... was cheating. Eventually I learned that it’s practical because there simply isn’t enough time to read...used both. Curate and present some useful data Typically lists and pictures are the most useful parts of...
  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...is simple -  no restriction, ligation or gel purification steps are required! Multiple fragment cloning...the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with...
  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... use is pX333 from the Ventura lab. pX333, a modification of pX330, contains humanized wtCas9 and two ...thus marking cells that have undergone genomic modification. In this system, Cas9 must be supplied on a ... combinatorial mammalian genetics.” Journal of Clinical Investigation 125(4) (2015):1603-19. PubMed PMID...
  9. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...
Showing: 761 - 770 of 884 results