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We narrowed to 772 results for: INA

Showing: 71 - 80 of 772 results

  1. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... “Three Es of Immunoediting” nomenclature. 1. Eliminate - Latent tumors are recognized by effector cells...interleukin (IL)-12 and interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous...in bacteria, the tumor cells that escape the elimination stage acquire genetic alterations and alter cell-surface...In contrast to the acute immune responses that eliminate early stage cancer cells, chronic inflammatory...tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment help...cells, and often cannot eradicate metastasis or eliminate the probability of a relapse. Chemical and radiation...to aide chemotherapy and result in successful elimination of certain tumors. However, antibody stability...

  2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...mRNA Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261...generally accepted that some form of homologous recombination (HR) occurs via this region of overlap, but ...single over-sized vector, but not always.  The combination of serotype and tissue/cell type could have a...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is...repairs Overview: The sequential homologous recombination approach delivers large donor templates using...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...Efficient mouse airway transduction following recombination between AAV vectors carrying parts of a larger...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...detailed instructions on how to generate the final recombinant adenoviral plasmid construct, please see Addgene...bacterial strain not prone to recombination for amplification. The final purified plasmid stock should...candidates for applications in oncolysis and vaccination!) In the 65 years since its discovery, researchers...linear double-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   ...to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated ...packaging cell line is transferred into the AdV by recombination. It is therefore recommended to test for the...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...

  4. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...bringing the C-terminal and N-terminal Cre fragments together and permitting recombinase activity. Opto-Cre-Vvd... of bacterial gene expression By Meghan Rego Recombinases are enzymes that recognize, cut, and alter specific...translocation or cassette exchange. By utilizing recombinases to manipulate genomes, scientists can control...expression of their genes of interest. While recombinases are powerful gene expression tools in and of...Cells expressing an RFP reporter plasmid for recombinase activity were exposed to blue light. Cre-Vvd ...substantially improved activity as compared to other recombinase/photodimer pairs. Created with BioRender.com ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

  5. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...situ of Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Purification in situ of Regulatory Elements) method. The original CAPTURE method was published in 2017 and addressed...by PCR or deep sequencing. A drawback of the original CAPTURE method is that a new cell line has to ... of the CAPTURE 2.0 system To improve upon the original CAPTURE method, the Xu Lab made the following ...changes to create CAPTURE 2.0:  Replace the biotinalatable tag on dCas9 with a BioTAP-tag. The BioTAP-...delivery of the two remaining CAPTURE 2.0 components eliminates the need to create stable cell lines and allows...

  6. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...peptide, while three bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka ...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907...Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] ..., 1984). The strategy they landed on was to recombinantly append their proteins of interest with unrelated...protein had a highly specific antibody to the C-terminal five amino acids, meaning the tag could be quite...). They noticed that Myc1-9E10 and the other C-terminal antibodies only recognized the human c-Myc protein...sequences in these species, they found that the N-terminal half of the antigen differs significantly between...

  7. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined recognition... with an inactive opsin gene; in Cre expressing cells, however, Cre induces the recombination of the double-floxed... P1 site-specific recombination. Purification and properties of the Cre recombinase protein." Journal ...Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant adeno-associated viruses (rAAVs) have become ...opposite orientations. Since Cre does not cause recombination between mismatched recognition sites, its presence...viruses, such as the optogenetic FLEx vectors in combination with transgenic animals or rAAVs expressing Cre... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...thereby rendering them inactive in the cytoplasm. The authors use PhoCl in combination with this caging technique...Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan...with nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3...Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving iPSCs (induced...discrepancies, which they traced to a 9 amino acid N-terminal variation in the Klf4 isoform (KLF4s or KLF4L)...variant they call mScarlet. Bindels et al used a combination of deliberate and random mutagenesis to isolate...light-dependent Gal4 transcriptional activation and Cre recombination. Find PhoCl plasmids at Addgene. Zhang W, et...

  9. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...today's biological research requires a close examination of specific proteins within a system. This can...expressed in frame on the N- or C-terminus of a recombinant protein of interest. The tag was developed as...wash steps removes excess proteins and other contaminants. The protein of interest is then eluted using...tagged protein of interest (purple) and several contaminating proteins (blue, green, orange) flows through...immobilized along with a polyhistidine containing contaminating protein (orange). A low imidazole wash buffer...buffer disrupts the weak interaction of the contaminating protein but not that of the protein of interest...wash buffer should be high enough to disrupt contaminant-metal ion binding without eluting the target ...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...cabinet, it needs to be decontaminated in a biosafety cabinet. When decontaminating liquid waste, remember...production), there is a significant risk for aerosol contamination. As most centrifugation has to be done outside...heat sealed tubes can be used for extra security. Finally, when spinning down lentivirus, you’ll need to ...you should open in the biosafety cabinet and decontaminate after use. Virus-exposed plastics and other ...other surfaces should be thoroughly decontaminated after use, typically with bleach (plastic) or ethanol (...cities may require a higher percentage of the decontaminate to be used for BSL-2 waste, and of course, if... percentage indicates the proportion of the decontaminate in the waste itself, not in the solution that...
Showing: 71 - 80 of 772 results