...exciting story I can tell with my data?  Can I make better slides that more effectively tell a story? Generally...publications (published, not in preparation) A history of successful funding (fellowship grants) A project...additional controls necessary? As you build your story, are additional data needed to rule out alternative...Before you do your first experiment in your new lab, stop and plan it out. Some questions to answer include...presentation. Yet, PFs need to tell and sell their stories to get jobs. PFs (and mentors) can never be too...question is yes. As you get better at telling your story, you can find clearer ways to consolidate or present...

...offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and we thought... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...in an automated manner. Addgene commonly uses a custom script derived from the count_spacers.py file from...representation of your library. When plotted as a histogram, the counts will give you a bell curve of the ...finds that this representation is most easily understood visually. As an example, the curves from our ...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

...widely varied with many properties, having well-understood standardized terms makes it a lot easier for ...plasmids at Addgene are shared under, is well-understood and accepted by the majority of research institutes...Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty years, we...your organization agrees to let Addgene receive, store, and distribute your material under the specified...move forward with shipping the material to the requestor.  Why the MTA?  The MTA process can be a bit confusing...exceptionally fast tech transfer offices with the Golden Stopwatch award and seek feedback as to how we can make ...

...help win a business plan competition and advance investors to the next stage of diligence." In business competitions...non-scientific audiences. "There are 100 ways to tell a story - convince your audience you are doing amazing things...The team is key, and the initial team is the cornerstone of it all. Without a good nucleus, the company...are 100X better than current technology and get customers to change behavior. When you are ready to commit...crowd funding, federal funding, foundations, and investors are examples of various sources of funding. Always... Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's Epiphany (or Lack ...

...privilege to find and share what stories we can, and to hear the stories others have to tell.  "Also thanks...we wanted to walk through some of our favorite milestones and share some thoughts from the community that...six years to reach our first major repository milestone — 10,000 plasmids deposited and available — and... which was a major barrier for depositors and requestors alike. We transformed this process by aligning...Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see the impact our repository...

... Outlines a detailed scope of specific aims, milestones, possible setbacks, and potential alternative...Carefully read over all parts until all aspects are understood. While reading the funding description, pay attention...will address these limitations and lead to new milestones in the field. Research strategy The Research...aims The Specific Aims section highlights each milestone that must be met in order to deem your proposal...proposals consist of 3 to 5 aims, with secondary milestones to meet along the way to achieving each aim. ...comments may make it clear that the reviewer misunderstood the point that was being made, take it as an...

...advocating-for-science-boston-2016/. Sign Up For the Advocating for Science Symposium Boston 2016 Changes since...Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science Symposium and Workshop...also started progress towards solutions. The 2015 Boston FoR meeting was designed to explore areas where...various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office...Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications...

.... The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid. B) Three...recommended to test for the presence of RCA in the viral stock by doing a plaque formation assay on A549 cells ...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction digest...you’ll produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the ...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification.... Purified high-titer rAdV is then dialyzed and stored at -80C. Purification and concentration kits that...

...cause bad reactions in mice. Customization AAV offers a level of customization that is unmatched by other...over the past decade. CRISPR screens are easily customizable and enable the targeting of hundreds to tens...lentivirus but offer an improved safety profile, customization, and enhanced expression in many mouse tissues...combination of serotypes and psuedotypes means you can customize your CRISPR screen to a high degree. This is particularly...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...

...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...their initial tests with thick slice (70 μm) immunohistochemistry (IHC) (Figure 5). PSD-95 is a scaffolding...