We narrowed to 884 results for: ica

...or DYKDDDK 1 Affinity and Purification Good for antibody-based purification; has inherent enterokinase...site GST Large Protein 26 Purification and Stability Good for purification with glutathione; protects...used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small...GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based purification Combo and cleavage tags...increasingly common. Tandem affinity purification (TAP) once referred specifically to a combo tag comprised of ...multitude of uses including (but not limited to) purification, detection, solubilization, localization, or...overexpress and purify proteins can face any number of technical challenges depending on their protein of interest...

...differing in pigmentation." Biochemical and biophysical research communications 78.1 (1977): 237-243. PubMed...one that can be achieved using pharmacological or electrophysiological methods (Deisseroth, 2011; Mei ...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry...Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural activity." Nature ..., et al. "Next-generation optical technologies for illuminating genetically targeted brain circuits." ...and Jinfang Zhu. "Immunologic applications of conditional gene modification technology in the mouse." Current... R. Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology...

...Spatially Confined Persistent Diradical Emitter. Journal of the American Chemical Society, 145(25), 13615–13622... tools for familiar applications. But how does one invent a tool for applications that don’t even exist...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible....of developing new microscopy tools for biological applications across a wide range of topics. He embraces...., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant to avian...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...

... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic... W. (2011). Chemical Tags for Labeling Proteins Inside Living Cells. Accounts of Chemical Research, 44...If you take a look in a typical lab freezer, you’ll probably find lots of different plasmids containing...new green fluorescent protein, some affinity purification tags, another version with that same red fluorescent...substrate. If the substrate was linked to, say, a chemical dye or other functional ligand, your protein of...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...

...of Various Motifs is Critical for Initiation of Replication. Journal of Biological Chemistry, 273(14), ...to utilize diverse host replication machinery. For instance, the ori typically contains iterons that serve...ori2, ori3) and replication initiation proteins (RepA, RepB) necessary for replication in various hosts...the ori and allow for replication. However, if plasmids have their own replication machinery such as Rep...different hosts that a plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow... while others have a broad host range and can replicate and stably maintain the genes they carry among.... These features are: The plasmid origin of replication (ori) contains structural elements that are versatile...

... your own research! Classification Structure Principles of CRISPR classification While CRISPR systems... CRISPR-Cas systems. Philosophical Transactions of the Royal Society B Biological Sciences, 374(1772),...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...properties. This led to a need to develop a classification system for the array of CRISPR technologies...technologies. Learning the general principles of this classification system will help you gain a deeper appreciation...multi-pronged analysis to sort effectively. The current classification system relies on a combination of sequence ...a similar function. After analysis, CRISPR classifications were placed into a fairly simple hierarchy:...

...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...systems consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with... as intact tissue. Some chemical labeling strategies such as the bisarsenical dye (Figure 1a) and FAP ...Ronald T. Raines. "Bright ideas for chemical biology."ACS chemical biology 3.3 (2008): 142-155. PubMed... at the Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is dead, long live chemistry...fluorescent protein (GFP) sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing...

...Retroviruses are not considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect...lentiviral delivery is not considered safe for clinical applications. Excisable Cre-lox lentiviral vectors:... to reprogram. Basically, there’s no universal method that can handle all applications of iPSCs, but there...generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming... to verify there’s no genomic integration Replicating EBNA1 episome Non-integrating Medium Medium ...creates heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing...unavoidable heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene...

... (enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136. PubMed...affinity purification with an anti-Cas9 antibody. These different tag systems and affinity purification schemes...26848818. Fujita, Toshitsugu, et al. "Identification of physical interactions between genomic regions ...genomic alterations. These studies utilize a catalytically inactive form of Cas9 known as dCas9 (Jinek ...interest targeted by a gRNA. CRISPR-mediated purification of a specific genomic region In 2013, the Hodaka...Subsequent mass spectroscopy studies allowed for the identification of proteins associated with the IRF-1 locus...steps (Fujita et al., 2013). CRISPR-mediated purification of a specific genomic region alleviates these...

...transfected (see Technical Tips). Minigenome replication and transcription In order to replicate the minigenome...transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems...allowing researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a ...that are crucial cis-acting signals for genome replication and viral gene transcription (6). The EBOV genome...5’ ends of the genome which are required for replication and transcription (Figure 1). All viral genes...cis-acting signals necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase... in 2, 5). The minigenome is capable of being replicated and transcribed in the cell when the appropriate...