We narrowed to 520 results for: Cit

...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...bright images of mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at...brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term...

...") and generally exuding a very-tired-but-very-excited attitude. Riding up to the resort in the shuttle...chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target effects....done with CRISPR technology in just 2 years and excitement about how much more there is left to do with ...

...regions are unique to each antibody and convey specificity to the target while constant regions are identical...resulting chimeric antibody retains the binding specificity to the original target and can be used with secondary...capabilities this process is frequently used to reduce immunogenicity and thereby enhance the therapeutic potential...

...School of Medicine. This deposit includes frequently cited antibodies for use in brain imaging (Andrews et ...recombinant antibodies are derived were tested for specificity in knock-out lines or animals.  Addgene will ... educational materials to the resource. We are excited to work with all of you to make this the best and...

...dimeric Keima is excited at 440 nm and in acidic environments at 586 nm. Regardless of excitation wavelength... emits at 620 nm. The ratio of signal strength excited at 586 nm to 440 nm serves as a measure of autophagy...

...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...to the research community.    So, why are we so excited? For starters, we believe that this approach can... for a host of other AAV serotypes; it will be exciting to test this approach with different capsids. ...

...on this topic, the authors state, “Both explicit and implicit aspects of today’s postdoctoral training...need to move around every 3-6 years—a short and exciting ride Complicated matrix structures are common...

...production to individual labs, but now we’re very excited to provide ready-to-use CRISPR lentiviral preps...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...lentivirus on our blog and protocol pages. We’re excited to provide this new service for researchers wanting...

...towards conjugates.  Storage   Once you get your exciting new antibody, the second thing you’ll want to ...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ... need to know the wavelength of laser needed to excite them - and it’s a good idea to make sure that laser...

...Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination...model for this study (Ref 6)). In addition, it was exciting to see that the use of optogenetic tools is expanding...observe the function of immune cells that are not excitable but responsive to calcium. We’d love to expand...

... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...

... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found that YFP and... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

...rate (Redfern et al., 1999) or altered neuron excitability (Zhu et al., 2014). The system allows you to...important to consider that the same receptor can elicit different responses depending on what cell type... chemogenetic receptors can be used to alter excitability by lowering or raising the threshold for signaling...

...antibody’s specificity. Strength and accuracy The strength is based on binding specificity and exposure...public service to the science community, and I am excited to be a part of it! If you are interested to get...

... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

...on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions...For applications that require high DNA editing specificity, for example for potential therapeutic approaches...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target...

... A major drawback of this method is the stray excitation of neighboring neurons in opsin dense regions...activity, and protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to...dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins have improved ...

...trials, wild type AAV is not associated with any pathogenicity and has a low immunogenic profile. AAV was first...new and refined forms of capsids with improved specificity. AAV vectors are able to facilitate and enhance...abilities to infect target tissues. Improved specificity through control of gene expression and the selection...

... the Science City The conference was located in the modern, relatively new “Science City” area on the...choose to locate there. I made time to go into the city of Guangzhou and see some of the sites. Most fascinating...