We narrowed to 365 results for: CRISPR

...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and gene expression...139-42. doi: 10.1038/nbt.3149. Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics...Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create a tool that is quick, versatile...robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the ...targeted to a very specific site on the genome via the CRISPR system. The ease and flexibility of the LACE technology...

...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...

...Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering ...more information on bacterial engineering using CRISPR on our blog). Her research should provide us with...

... Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...State University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids...

...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by ...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...Prime Editing: Adding Precision and Flexibility to CRISPR Editing PRIDICT: Predicting Efficiencies of Prime...

...Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical...transgene-expressing or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods...Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out...including Addgene’s top requested plasmid in 2015: lentiCRISPR v2, used both for targeted modification and genome-wide...

...Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu...'s lab at Peking University has developed a new CRISPR screening method that makes use of guide RNAs with...false-positive rate discovery rate, conventional CRISPR screens require a low multiplicity of infection...

...Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation CRISPR 101: Epigenetics and Editing...Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR Between the Genes: How to Experiment...or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how does this...

...function of tagged proteins and facilitates precise CRISPR-based tagging, or low-levels of ectopic expression...range of workflows, from classic overexpression to CRISPR knock-in, letting you track endogenous protein ...detail on whether the authors incorporated HiBiT by CRISPR tagging at the endogenous locus or used other expression...kinase essential for cell proliferation signaling. CRISPR knock-in HiBiT-tagged EGFR cells allowed researchers...Illuminating Transmembrane Proteins Using HiBiT CRISPR Cell Lines. Promega Notes. https://www.promega....illuminating-transmembrane-proteins-using-hibit-crispr-cell-lines/ Shibata, C., Otsuka, M., Shimizu, T...

...piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ... to the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations...interest, along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized ...

...Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference in...Research Institute. When it comes to genome editing, CRISPR is a name that resonates with nearly every biologist...academic, and researcher. Among the most well-known CRISPR-associated nucleases are Cas9 and Cas12a, the heavyweights... Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...

...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...

...assemble and test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, ...

...morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this... Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs...Use Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...

...about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens Using...

...blog: Learn how the CRISPR software matchmaker can help you choose the best CRISPR software for your needs...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell...

...induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and ...functional role of those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse...

...genomes using exogenous enzymes: Lambda red and CRISPR Developed over the past 20 years, Lambda Red recombineering...used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation ... "RNA-guided editing of bacterial genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): ...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables...blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...

... to. When it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred...1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker and the repositories...vast and difficult to beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose..., it was the go-to choice for many years before CRISPR burst on to the scene. shRNAs reliably knock down...in Plant Biology: New Tools for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids ...

...101 blog series Learn more about CRISPR and genome editing in our CRISPR 101 blog series Resources on Addgene.org... in gene modification tools and methods such as CRISPR have vastly improved scientists’ ability to use...use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus...Xie, Z., Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional...

...Addgene Blog Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture...; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts of...the 48-well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western...

...xCas9, a CRISPR variant with PAM flexibility Resources on Addgene.org Get the basics of CRISPR in our ...our CRISPR guide Find all plasmids for base editing in our collection Sign up to get our free CRISPR eBook...

...Your gRNA for CRISPR Genome Editing PRIME Editing: Adding Precision and Flexibility to CRISPR Editing Resources...Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...

...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite... Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection Read our molecular biology reference...

...plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making...localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab...

...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...

...Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed nearly 2,400 citations in its first...much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers and Addgene deposits, but papers published... Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (2013): 819-823....

...technique is also highly relevant in this age of CRISPR; site-directed mutagenesis generally applies to...DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed...a similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...More about Plasmids in Our Plasmids 101 Series Use CRISPR to Generate Specific Genomic Mutations Resources...

...Architect Pam McA' Nulty (check out our blog post on CRISPR software to see the importance of programming for...Scientist Joel McDade’s presentation on the emerging CRISPR genome editing technology was a rousing success...

... infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited...