...nowadays, we cannot make it a full month without a new, drug-resistant ‘superbug’ making headlines in one... develop survival mechanisms. Some microbes even make their own antibiotic compounds. In such a case, ...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...

... for talk is over and instead we must make real changes to make the system sustainable. For years, there...Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed...opportunity to accept these recommendations, and make gradual adjustments towards this new level. Indeed...

...advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has...many available through Addgene’s repository) and make it easy to find your perfect match. Choosing a ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...

...aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor/multichannel...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging experiments...Sample preparation for these techniques sometimes makes it more difficult to detect protein tags using antibodies...Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for...

...preference, some people like to make stricter gates while some like to make looser ones (Figure 3). A more...free to use SSC for a single cell gate. However, make sure to compare H and A (or W and A).   Live... type of stain whether a tighter or looser gate makes sense. Here, luckily, the vast majority of my cells...

...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...months. But certain models remain more difficult to make. For example, large insertions, like a fluorescent... site. If you are using the ivTRT method, try to make the first two bases of the left homology arm guanine...activity. To avoid re-cutting by Cas9 after editing, make sure that the repair template does not contain the...

...larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of large populations...backbones designed right here at Addgene. Here's what makes these new backbones special: Strong expression:...Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular by design...recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base changes...

...used for delivering your light stimulus. This can make or break your experiments!  First, closely read ....2021.778900 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L... B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J...science.aaw5202 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L...

... of DNA as inert. It generally stays put, which makes it easy to locate in a genome. But there is a type...genomes of many other prokaryotes and eukaryotes, and make up ~45% of the human genome (Munoz-Lopez et al.,...considered replicative since every time they jump they make a copy of themselves. This increases the number ... by their nature, are mutagenic elements, which makes them a great tool for mutagenesis screens that detect...

...you get the idea. What if there were a way to just make one fusion protein that switches to any color or...experiment (Figure 3). And using self-labeling tags makes updating your labels easy! Instead of taking the...ligands is more expensive than letting the cells make a fluorescent protein for you. And some ligands ...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...

...non-dividing cells, including stem cells. These properties make lentiviral vectors fantastic options for delivering...such as macrophages and microglia. This property makes the lentivirus an attractive choice for a gene transfer...the accessory factors in HIV positive cells could make the virus replication competent. The 3rd generation...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

...differentially expressed genes can be the next stage in making sense of such data. These targeted screens can ...you have your list of genes, Green Listed can help make the gRNA design process quick and easy. Green Listed...information that you have input in the web interface, making it convenient for you to later identify how you...letting us use their reference libraries thereby making it possible to put together this software. To me...

...you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow to Make Friends and Meet...Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and Accessible Scientific...

...taking care at each step, but some purification kits make it easier than others to prevent quality issues....miniprep kits! Larger kits generally take longer.) Make sure to take a look at the manufacturer’s kit pages...select a kit. So...which kit should you pick? To make it easier to compare kits at a quick glance, Table...budget is an important consideration, you do want to make sure you’re getting the best bang for your buck....

...target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA...the core ring structure intact, allowing them to make 4 out of the possible 12 base-to-base conversions...were engineering an ABE to reduce its tendency to make off-target edits to RNA. Like other ABEs, the editor...mammalian cells Researchers in the Xueli Zhang lab were making similar observations around the same time. (The...

...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com... wrapped gifts using life science catalog pages. Make your WFH space feel a little more festive this holiday... entering in (if you don’t include this, we will make our best guess). Follow @Addgene, use the hashtag...

...experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical Addgene badge...favorite topic. If you have any other cool tricks to make PubMed browsing even easier and more useful, let...

...recent blog post, we looked at how long it takes to make one plasmid and how much time Addgene has saved ...researchers. Assuming 5 hours of hands-on time to make each plasmid, we would have saved 5 million hours...will be moving into a bigger space so that we can make an even bigger impact - we are always on the lookout...

...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...

...PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your... and the small difference in protein expression makes confirmation of antibody selectivity even more challenging...used has endogenous levels of expression, this can make the results more difficult to interpret, and a knockout...Antibodies. The Only Good Antibodies community works to make best practices in research antibody selection and...

...Neuroscience meeting in Washington, D.C., we must be making a dent in the global coffee supply… My back-of-...we've spoken with are excited and passionate about making such tools open-access. Though it is time for me..., remember there are only 2 days left for you to make the most of this great opportunity – so if you’re...

...capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic cassette that...Another challenge is that multiple transfection makes it difficult to control the dose of plasmid the ... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

...is being an overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...additional person each day. It felt like I could actually make connections with people. These were some of the ...

...control of an otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled...paired with any Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1...other viral vector tools in the years to come. We’ll make many more viral vector tools and resources available...

...story. This year, we made significant strides in making science more accessible and reliable. We continue...experimental results. We also redesigned our website to make it easier to find what you need and to bring it ... every breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank...

...expression vector and attached a DNA barcode to each to make the library usable for pooled screens. Applications...different contexts.” It’s also possible you could make use of these plasmids in living animals, to explore... that’s thrilling to us,” Wood says. “We want to make these available as widely as possible.” The researchers...

...this interest in the ways science is shared and in making it easier to share, we take special note as the...Way to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As... As we prepare to ring in the New Year, let's make a toast to open science. And, please, share your favorite...

... the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative...derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs)...the unexpectedly photoconvertible GFP proteins to make a new transgenic line could avoid problems in localization...

...partners and Tech Transfer Offices at universities to make COVID-19 research-related plasmids available more...Find plasmids available to industry scientists To make plasmid sharing more open for academic and industry... deposited plasmids at Addgene and would like to make them available to industry, please reach out at ...

... by making them brighter, more photostable, smaller, and less toxic to cells. They’d like to make near-infrared... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...