960 results

Addgene supports rare disease research with the 2020 BeHEARD Awards. Read about the winners and their research in this blog post.

Sept. 8, 2020, 2 p.m.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Sept. 3, 2020, 12:15 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

Aug. 27, 2020, 1:15 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Aug. 18, 2020, 1:15 p.m.

Addgene marketing intern, Brandon Solis, shares his experience working virtually in a COVID-19 world and his Meet an Addgenie Instagram project.

Aug. 13, 2020, 1:21 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

Aug. 4, 2020, 1:15 p.m.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

July 31, 2020, 12:30 p.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.

Addgene's new cloning grade DNA service allows you to start cloning right when you receive the plasmids from Addgene. No amplification and extractions necessary!

July 28, 2020, 1:15 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Find details on five methods to quantify DNA: UV absorbance, fluorescence dyes, agarose gel electrophoresis, capillary electrophoresis, and diphenylamine.

July 21, 2020, 1:15 p.m.

David Liu's lab analyzed how COVID-19 affected their plasmid distribution.

July 16, 2020, 1:15 p.m.

Find seven tips for scientists when preparing for an industry interview, including advice for remote interviewing.

July 9, 2020, 1:15 p.m.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

July 7, 2020, 1:15 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Find tips for getting the best virtual conference experience from attending talks, poster sessions, networking, and taking advantage of the virtual format.

June 30, 2020, 1:15 p.m.

Our new Instagram series, Meet an Addgenie, features stories form Addgenies about their career decisions, paths, and more.

June 25, 2020, 1:15 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.

Addgene expands plasmid distribution to industry scientists during COVID-19 and beyond. Learn more about the collection in this blog post.

June 23, 2020, 1:15 p.m.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

June 18, 2020, 1:15 p.m.

Using plasmids for transformation, cloning, or transfection? Different uses mean you'll need different purities of DNA for your applications.

June 16, 2020, 1:25 p.m.

So you want to control neuronal activity, but should you choose chemogenetics or optogenetics? Read this article to see how these methods compare.

June 11, 2020, 1:34 p.m.

Tris? Tris-EDTA? EB? There's many options for eluting plasmid preps. Find out how elution and storage temperature affects DNA stability and downstream applications.

June 4, 2020, 1:22 p.m.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

June 2, 2020, 1:15 p.m.

Learn about items from Addgene's viral service including lentiviral preps expressing SARS-CoV-2 proteins, ChR2 for targeted expression, DREADDs, GFP dependent Cre and FLPo, and more.

May 28, 2020, 1:15 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

May 21, 2020, 1:15 p.m.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

May 19, 2020, 1:15 p.m.

Find a list of databases and resources for COVID-19 research and hear from the creators of these databases.

May 12, 2020, 1:15 p.m.

Scientists at Beam Therapeutics have found a way to improve editing in primary human cells using evolved adenine base editors called ABE8.

May 7, 2020, 1:15 p.m.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

May 5, 2020, 1:15 p.m.

Addgene welcomes three new members to the Board of DIrectors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe.

April 28, 2020, 1:14 p.m.

Viral genome sequencing is a high-throughput method for determining AAV transgene identity and confirming the serotype.

April 23, 2020, 1:15 p.m.

Find tips for remote collaborations including scheduling regular meetings, maintaining informal communication, sharing documents, setting expectations, and meeting in person.

April 21, 2020, 1:14 p.m.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

April 16, 2020, 1 p.m.

Scientists from UCSF identify 332 human proteins that the SARS-CoV-2 virus interacts with in cells in attempts to find druggable targets.

April 9, 2020, 1:15 p.m.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

April 2, 2020, 6:17 p.m.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

March 31, 2020, 1:15 p.m.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

March 24, 2020, 1:15 p.m.

New to remote working? Find tips for self-care, professional development, writing, balancing working from home with parenting, and more.

March 19, 2020, 1:15 p.m.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

March 17, 2020, 1:32 p.m.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

March 12, 2020, 1:15 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

March 10, 2020, 1:15 p.m.

Addgene's AAV Data Hub is a place for researchers to find and share experimental parameters of using AAV. such as titer, injection site, and more.

March 5, 2020, 2:15 p.m.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

March 3, 2020, 2:15 p.m.

Learn how to place an order using a PO number for your Addgene order.

Feb. 27, 2020, 2:15 p.m.

Take a look at the developments in genome engineering throughout history and the parallels in science fiction.

Feb. 25, 2020, 2:15 p.m.