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We narrowed to 812 results for: APP

Showing: 351 - 400 of 812 results

  1. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...toward the cathode (- charge). When the voltage is applied across the gel, the DNA migrates toward the anode...want a “recipient plasmid : insert ratio” of approximately 1:3. Since the number of base pairs for each...should also verify that you are plating on the appropriate antibiotic and try varying the “recipient plasmid...Ideally, once you know that your plasmid has an appropriately sized insert, you should send it for sanger ...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...cells with no Cas9 should appear green and cells with functional Cas9 should appear less green. Note that ...the population, so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips...by the presence of DNA cleavage products. The appearance of the 428 bp band indicates edits in the BRAF...

  3. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve learned about...and Tol2), each of which has several different applications (mutagenesis screens, transgenic animals, gene...Garcia-Perez J (2010) DNA Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/...Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome...

  4. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

  5. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...Shakespeare for the first time. The discovery and application of CRISPR-Cas9 kicked off over a decade of frenzied...classification system will help you gain a deeper appreciation and understanding of CRISPR, and perhaps provide...mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://...Mashimo, T. (2022b). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...protein composition. It is used for a variety of applications such as measuring target protein expression ...stain the intracellular target. When using this approach, avoid fixation-sensitive fluorochromes such as...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...antibodies during the flow cytometry experiment and will appear as a distinct population. After initially gating...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...PAM is so flexible in this state that it doesn’t appear in the crystal structure (Figure 2) at all. When...cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure 3 crystal structure, reflecting the...which stabilizes the last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where...with and better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed...

  9. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...show the interactions between amino acids in an approximate, two-dimensional map. The Baker Lab wanted to...novel, the principles that created it have been applied to numerous other protein design goals, both from...drugs, all developed with their protein design approach. Congratulations to David Baker, Demis Hassabis...

  10. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...This edition of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes...piece of advice - get your PI or lab manager’s approval before letting your imagination run loose on the...diorama. Or maybe you want to create a stack of wrapped gifts using life science catalog pages. Make your...

  11. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...this post to anyone wondering if and how they can apply FRET in their own research. All posts from July ...Blogger Form and we'll be in contact with you ASAP. Happy Reading! Tyler ...

  12. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...regions in the near future. Additional Resources Apply to Recieve Equipment through Seeding Labs' Instrumental...

  13. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...brains on how they've managed to keep themselves happy during their time in grad school. As you'll learn...student communities 5:59 - 8:16 : What has made you unhappy in grad school? As part of this discussion, we ...to the right that illustrate a grad student's happiness v time throughout graduate school. This is, of...

  14. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ...a plasmid or viral origin. The type of cloning approach you choose ultimately will influence the quantity... for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms or ...assembly or Golden gate assembly use a more direct approach, allowing scientists to clone from a smaller amount...

  15. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...Citing This Plasmid" database - and we always appreciate any help we can get. If you know of an article...PMID) for the article that has used this plasmid. Happy researching! If you need any assistance finding ...have made to the repository, email us and we'd be happy to help. Read More Tips from Addgene Plasmids ...

  16. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...looked at the trace you would be happy. But look closer, the overlapping peaks in the chromatogram suggest... or consider asking your sequencing facility to apply a special protocol for a difficult template. Anything...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic...feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking for CRISPR...

  18. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could...don’t think you’re qualified. The worst that can happen is they say no, which is infinitely better than...good, customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there...

  19. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... make the library even better, we would be very happy to share updated versions of the kit.” Reference...

  20. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to prepare....org.  Thank you again for a fantastic year and happy reading. Best wishes on your research in 2020! ...

  21. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    Published
    Sept. 9, 2016, 2:43 p.m.
    ... huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome library...already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the 70,000 or more...community will find these reagents to be useful. Happy screening! Many thanks to our guest blogger, John...

  22. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...need for a faster and higher-throughput in vitro approach. Using data from The Cancer Genome Atlas (TCGA...and fusions represented in this collection, approximately 80% (over 800) of them had not yet been functionally... viability compared to a wild-type control. Approximately ~22% of the mutations, however, significantly...

  23. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...ve also carved pumpkins (yes, Blugene made an appearance there too) and shared fascinatingly creepy science...defective exoskeleton formations. These genes were appropriately named as spook, spookier, phantom, disembodied... grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B: Biological...

  24. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh...

  25. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... size and frequency [3]. There are several applications in which the production of deletions by paired... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  26. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...were willing and able to listen to Addgene’s new approach of working with the industry-based scientific ...reduced to days and weeks. As of June 2020, we are happy to have the support of the following organizations...project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research...

  27. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...opportunities.  Scientists must do science to be happy so the beginnings of solutions can’t come too soon...

  28. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...expression level. Labs have been trying different approaches to improve CTC capture, but I hope that our antibody...

  29. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...go-to method for mapping where a protein binds genome-wide, and has been widely applied in many model organisms... the chance of false-positives, as proteins may appear to be binding at particular genomic sites when ...

  30. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  31. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...been requested 100+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing...plasmids with us. One way we can gauge if people are happy depositors is if they deposit plasmids more than...off! To share your photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a ...

  32. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...inflammation, and neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda...GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs. Cells are then treated...plasmids in a number of different experimental approaches. They first assessed the activation of different...

  33. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...called protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing... are recognized by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or ...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  34. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ... al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...population, it’s easier to visualize individual/non-overlapping cells. While transgenic animals are commonly ...

  35. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware...

  36. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

  37. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...team. It is pretty clear that if people are mostly happy at work, they will work harder and contribute more...contribute to job satisfaction: A feeling of being appreciated and valued This often stems from being included... Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental...

  38. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has ...I started looking for smaller and smaller gel apparatus until I settled on using glass slides. With just...this one only cost a few dollars.  I used this apparatus for 15 years before I hung up my pipets.   ...

  39. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ...Review and Scientific Publication, where diverse approaches to peer review were discussed. Science communication...

  40. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which...

  41. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein College ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action... to your DNA sequence of interest. Potential applications of the system include activation and repression...

  42. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...chatting makes it pretty easy to make 30 minute appointments with people all over the world. Think about ...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get...

  43. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  44. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...form has 5 general question types and it takes approximately 15 minutes or less if you have all of your data...under copyright, you will need to obtain written approval from the copyright owner and will have the opportunity...viewing the data you have any feedback at all, we’d appreciate you sharing it through this form. In honor of...

  45. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...113049 offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h...

  46. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed...

  47. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...your meetings productive: Discuss logistics if appropriate:  How often will you meet? How does the Mentor...the process of working on them is where growth happens. Mentors bring more than their knowledge and teaching... get a bunch of text messages asking me, “What happened? Where are you?” My gym posse holds me accountable...

  48. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... the self-assembling capability of sfCherry approximately 20 fold by directed evolution. They then optimized...concept that the designed used in FlipGFP can be applied to other fluorescent proteins to create an array...and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage...

  49. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

  50. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...actually making it happen would increase dramatically. I’m not sure what the best approach to doing that will... communication about experimental methods and approaches. It’s very easy to think that the methods section...
Showing: 351 - 400 of 812 results