We narrowed to 868 results for: REN

...sensors with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although...Improving on the glutamate sensor iGluSnFR from Loren Looger’s lab, Kaspar Podgorski and colleagues have...response. They generated variants optimized for different applications (v82 has higher affinity for glutamate...By fusing two bright fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato...sub-millisecond resolution and sub-degree temperature differences under a variety of imaging conditions (155 μs...

...solubility, and thus different reagents are useful for biotinylation in different microenvironments. There...used today in molecular biology for an array of different techniques and protocols. In this post we will...determined to be due to a deficiency in Vitamin H later renamed Vitamin B7/biotin. Egg white injury would plague...a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b): 359–363....

...scientists have developed and fine tuned many different ways to clone DNA fragments which have provided...with an enzyme master mix, which contains three different enzymes: an exonuclease, which chews back the... this doesn’t allow for mix and match of many different fragments. One way around this is to use a combination...with a G!) but actually work by significantly different methods. Golden Gate cloning does result in the...from one vector to another. There are lots of different ways to clone these days. Gibson and the other...biology, you should consider giving it a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC,...

...available at Addgene CRISPR-Cas9 References, Protocols, and more References: Generation of Gene-Modified ...the monkey story hit the presses, another very different bit of CRISPR news was circulating. Scientists...how CRISPR/Cas9s actually work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna and Eva Nogales...this structure may help us engineer around the current limitations of the Cas9 complex," said study author...

...localization signal; TEVp = TEV protease. Created with BioRender.com. Using this system, the team tracked the...it is a diverse tool that can be used in many different systems, both in vitro and in vivo. Find MitoTRACER...Fang, et al., 2025). These new sensors use quite different designs, leading to unique advantages. While most...most red GECIs have been derived from just a few parent fluorescent proteins, PinkyCaMP is the first based...transplanted into the vicinity of any one of five(!) different cancer types, the tumor size was greatly diminished... & Lau et al., 2024). The method uses CRISPR interference (CRISPRi) to regulate gene expression of colorful...

...by Promega scientists here.  References and resources References Hall, M. P., Unch, J., Binkowski,...overexpression — boosting levels so interactions aren't missed. But this often yields results that don'... al., 2018). The small size of HiBiT reduces interference with the native function of tagged proteins ... regulation, and context-dependent activity can render traditional tagging approaches insufficient. Vascular...., Toyooka, S., & Otsuka, M. (2025). Genomic Differences and Distinct TP53 Mutation Site-Linked Chemosensitivity...

...by Hannah Dotson in Dec. 2022.  References and Resources References Gossen M, Bujard H. Tight control...In the presence of tetracycline (Tet), TetR preferentially binds Tet over the TetO elements, allowing ... One can therefore place GAL4 and GAL80 under different promoters and create sophisticated patterns of...wondering, “why is it necessary to have all 3 of the different binary systems above if they all behave very similarly...tet-on-and-tet-off-2nd-generation Mullick, A., Xu, Y., Warren, R. et al. The cumate gene-switch: a system for...

...line with the piggyBac™ system is that it has a preference for integration at regions of open chromatin,...rearrangement (such as when a stem cell initiates differentiation), transgene silencing may occur (as it would...cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR assay...technologies and strategies for their projects. References 1. L. E. Woodard and M. H. Wilson, “piggyBac-...piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no. 9, pp. 525–33, Sep. 2015...26299960. PubMed Central PMCID: PMC4559351. 8. A. G. Uren, H. Mikkers, J. Kool, L. van der Weyden, A. H. Lund...

...Toronto, created The Addictive Brain to spread awareness about how scientists utilize tax-payer money and... how our articles have helped them understand different topics of science, especially disease related ...terms like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate...blogger, Chinmaya Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the University of Toronto and founder... receive our science career guide Learn about different careers in science communication Resources on...

...fragments that are very different.” Because the fragments produced will have different overhangs, they can...systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann...Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target...available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos et al. "GreenGate ...

...5,000 different research institutions. This has given us a unique window into technology trends in the...by the influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids...HA-Ubiquitin-WT and HA-Ubiquitin, and we now have 19 different ubiquitin plasmids in the repository. 100,000 ... and modified by so many labs and for so many different purposes that we had to create a CRISPR page to...

...controlling properties like growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs...implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For each of these 17 pathways...morphological features, migration, or survival in different contexts.” It’s also possible you could make use... sometimes drug screens and sometimes totally different experiments we never thought of - and that’s thrilling...constructs to the kit in the next two years. References 1. Martz, Colin A., et al. "Systematic identification...

...tools to the expanding gateway compatible vectors currently shared amongst the zebrafish community. Don E...PiggyBac transposons, the Woltjen Lab compared different variants of the polycistronic cassettes and discovered...is a new member of this class that works via a different mechanism - the PhoCl protein is irreversibly ...“caged” by fusion to specific domains thereby rendering them inactive in the cytoplasm. The authors use...interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain -...of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown ...examining genomes possessing CRISPR systems with apparent self-targeting. Two of these Lmo proteins, AcrIIA2...

... Conversations with children playing in the background became a normal occurrence as Addgene and our university...light of the need for basic research during the current pandemic, universities are showing an unprecedented...of Illinois Chicago                     Boston Children's Hospital                           California...scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure allowed them ...

...year bioengineering student, to Addgene. I am currently working on the QC/QA team as a DNA Sequencing ... food service industry, which followed a very different schedule. Fortunately, I was provided with a detailed...gave me enough time to become familiar with the different types of issues that were important to this function...Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At this point...point, I have seen several lab processes and am currently working on projects for the Science Communication...

...neuronal populations to consider. To tease apart how different neurons impact a certain output, neuroscientists... populations using viral strategies Combining different approaches can increase the specificity of cell...optogenetic payload in neurons that project to different regions of the brain. A more locally defined set...in their cell bodies. Optical stimulation in a different brain area can then reveal the cells bodies of...share experimental design and data. With these different approaches to open science, we aim to accelerate...

...NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three colors. The...specificity of these orthologs is key: due to differences in the PAM sequences required by each ortholog...specific for telomeric sequences and showed that different fluorescently labeled dCas9 isoforms are efficiently...target sequence. They were also able to label two different pairs of chromosomes using gRNAs specific to sequences...Addgene are excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci...

...trained to be good at learning new things—this is a strength you can draw on and demonstrate in your application...be excited to be there. If you are appying for diffrent types of positions (for example, bench research...resume should also be customized to highlight different skills and to get the important information in...hiring manager. Learn to confidently describe your strengths in your resume and don't be shy about asking for..., you will never get the help that can make a difference. Having your resume forwarded personally gives...

... Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection...together genes from gas vesicle operons from two different bacteria, Bourdeau et al created two constructs... 1 (arg1) and acoustic gene 2 (arg2). The key difference between arg1 and arg2 vesicles is the amount ... invisible to ultrasound, which allows for differentiation of arg1 and arg2 ultrasound signals. This also...and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A., Lakshmanan...

...and insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides...Development of a bacterial expression system for renewable biotechnology products Dr. Idah Sithole-Niang,...database for this important agricultural plant. Her current research focuses on endophytes, bacteria, and fungi...effect observed in bacteria. Learn more about the different CRISPR applications in plants.     Transgenic ...helped us coordinate and promote the awards. References 1. Martinez-Klimova, Elena et al. “Endophytes...

...antibodies, and more!   Resources and References   References Coligan, J. E., Dunn, B. M., Speicher, ...Nonetheless, this tag can be purified with several different resin types and is a fairly small tag that is ...  Affinity tags vs. other tags There are many different types of tags, including epitope tags (FLAG, Myc..., D. W., & Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein Science. John Wiley & Sons, Inc...

.... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...and co-founder, approached me and asked about my current projects and personal interests. We chatted about...participated in the discussions, I began to note trends in the data, and from that, suggest changes to ...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ...

... tissue, in vivo. Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral ...have high selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1...two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than HcKCR2, making... be used as a potent optogenetic silencer. The current work also proposes promising avenues for future...anti-c-Myc [9E10] (isotype IgG2a) antibody is currently recommended for use in western blot, but we are...

...leader-specific. How is leadership different from good management? Aren't all career skills leaderhsip skills...because it emphasizes the importance of these very different roles. At any stage in our careers we may be in...you are not fulfilling the requirements of the current task. Most of us start as doers, some become managers...boss) that you are ready to try operating in a different role?  I think the secret sauce can be summed ...

...due to genetic drift and the use of genes from different microbial strains. It proved to be impractical... least 96% sequence identity when detecting a reference feature. For a coding sequence feature that may... could miss a common feature if the sequence differences exceed the threshold. This issue is addressed...often come in closely related versions that have different properties. To prevent misidentification, we augment...way for SnapGene users to apply features from a reference strain to newly sequenced strains through our ...

...cells. The replicons of plasmids are generally different from the those used to replicate the host's chromosomal...include ColE1, pMB1 (which comes in a few slightly different but well known derivatives), pSC101, R6K, and ...considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived ori that allows for...chloramphenicol – make sure your growth conditions aren't working against you!  The culture inoculum ...clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, Xiao H, Schultz...

...AAV. You’ve never worked with AAV before, but you aren’t going to let that stop you. Where do you start?...capacity between the ITRs is ~4.4 kb. ITRs are inherently unstable due to their secondary structure, palindromic...species or location. Promoters can also drive different levels of expression. For example, CAG is a strong...Next let’s talk about some of the elements that aren’t required parts of an AAV transfer plasmid, but ...specific expression of your gene of interest. References Haery L, Deverman BE, Matho KS, Cetin A, Woodard...

...the issue is not unique to IHC.  References and Resources References Goodpaster T, Randolph-Habecker J...endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding background secondary...them to your sample so the secondary antibodies aren’t free to bind endogenous IgGs. (C) A third solution...primary and secondary antibodies. Created with biorender.com. Proper tissue processing and sample preparation...

...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...electrophysiological potential afterwards. This measurement trended with expression of the opsin in the target neurons...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...

...obstacles that need to be overcome. Conflict, and its inherent tension, is interesting. It’s what drives a story...actually from a completely unexpected finding in a different experiment, this tool was developed because a ...couldn’t progress without it… These details often aren’t included in scientific communications, but they...scientists doing the work.  And while these pieces aren't always appropriate for your formal scientific communications...doing and sharing science in the first place?   References and Resources More resources on the Addgene blog...

... our values of transparency, reproducibility, and community-building. The transparency begins with sharing...whether any given antibody is available through different companies (Bandrowski et al., 2016). It’s also... the antibody sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT... Improving Reproducibility through Rigor and Transparency of Experimental Methods. Neuron 90(3):434-6....

...previously hidden epitope (right). Created withBioRender.com.   Biological Processes  If you’re feeling...outside of the cell. The same protein may have different epitopes available if it is being selected out...controls in validation, as you may need to test different configurations and conditions to understand exactly... what a given protein is doing or changing in different conditions. Just remember to keep good records...when you’re building your antibody library! References and Resources More resources on Addgene’s blog...

...antibodies for tagging purposes. Different antibodies bind different protein sequences called epitopes...infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements...living cells. To make these scaffolds specific to different targets, the scientists fused them to sequences...to build a more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004...

...Argonaute proteins play an important role in RNA interference, binding RNA guides to cleave foreign RNA. Argonaute...guides/gene for 10 genes, they did not observe differences in cleavage efficiency. If this finding is replicated...) or staggered (Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from...Read About NgAgo User's First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang... 2. Swarts, Daan C., et al.  “DNA-guided DNA interference by a prokaryotic Argonaute.” Nature. 507(7491...

...transition from a clump of cells to a fully differentiated body. In contrast, these dynamic processes ...distinguishing cell populations that have been tagged with different fluorescent proteins or dyes. 3. Two-photon microscopy...cells. For example, by harvesting lymph nodes at different times after antigen challenge, scientists were... see movement and interaction between several different immune cells. These methods allowed immunologists... like this is a mosaic of cells labelled with different fluorescent proteins. Using one iteration of this...understanding of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging...

...isotype is expressed at different times of the immune response and initiates different immune cascades. In...In the research setting, antibodies of different isotypes can be used together in the same experiment ...However, each animal will make antibodies against different protein epitopes and so variability between lots...types of antibodies are out there, and how many different research applications they can be used for. Addgene... - and we’re always growing our collection! References: Arbabi-Ghahroudi M (2017) Camelid Single-Domain...

...globe, it can be a bit of a mystery for those who aren’t familiar with it. If you’re curious about the hows...is shared between two parties. There are many different flavors of MTAs, but the Uniform Biological Material...which sometimes makes people think our MTAs are different than other institutes. But they’re not! The vast...perfect, but it does provide consistency and transparency in an otherwise messy process.  And you do have..., instead of coordinating signatures from two different institutes. Our online portal automates a lot ...

... the library are differently sized, smaller plasmids may be amplified preferentially, skewing the composition...successful amplification? To provide a point of reference, Addgene commonly prepares NGS samples using both...overrepresented plasmids (plasmids that were amplified preferentially). You can also rank the plasmids by abundance...create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the area under the curve ...

...have risen into yeast expertise! References and Resources References Liu, Wei, et al., From Saccharomyces...lists some of the biological commonalities and differences between K. phaffii and other yeast strains.   ...Applications for K. phaffii There’s a number of different uses for the phaffii-lous strain. Protein expression...The yeast giants - S. cerevisiae and S. pombe - aren’t going anywhere anytime soon, and for good reason...

...that replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger...intended to help early career scientists attend a conference of their choice to share their fluorescent protein...are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K epoxide reductase...and rapidly test the variants in vitro against different drugs and dosages.  Michal Bernach: Developing...

...for creative interaction During Zoom meetings, different tools can be used to promote creative interaction...as a picture. I believe that showing something different from time to time breaks up the monologue a little...papers will be written by several authors from different institutes, disciplines, and cultural backgrounds...and only general chatter will be allowed with a different host each time that moderates conversations. This...communities. You can follow him on twitter @salmon_phd References Frassl MA, Hamilton DP, Denfeld BA, de Eyto E...

...based on the predicted sizes and organization of different features within the plasmid. Restriction analysis...can search NCBI for YGOI to find the particular reference sequence if necessary. The example plasmid on ...fragment having increased intensity to serve as a reference band. The uncut DNA (lane 2) shows 3 possible ...in the gel well and the dyes provide a visual reference point so you can easily assess how far the gel...Eductional Resources: Addgene's Molecular Biology Reference Find More Protocols and Videos      ...

...Sanders of ComSciCon, the Communicating Science Conference series for graduate students. I believe that...giving a public talk, ask a friend who works in a different field to listen to you practice.  Ask them what...organizations. At ComSciCon, our primary mission is to strengthen and support the network of graduate students ...addition to this annual event, graduate students in different regional and disciplinary communities gather at...Learn about careers in science communication The strength of story telling Science communication: 9 strategies...

...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin...Architecture and Chromosome Organization. These aren’t the only investigators in the chromatin field who... Note that histone genes frequently have many different variant names, not all of which can be listed ...Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody...me at the Keystone Chromatin meeting, especially Karen Reddy (@theReddyLab), Thoru Pederson, Joanna Wysocka...

...far the T4 exonuclease proceeds: at the first occurrence of the chosen nucleotide, T4 will switch to polymerizing...efficiently repair the plasmid. Created with BioRender.com.   SLIC also opened up a new world of multicomponent...bacteria, which repair the plasmid. Created with BioRender.com. How does SLIC compare to other cloning ...method’s flexibility allows it to be used with different types of PCR-generated inserts. By harnessing ...updated by Emily P. Bentley in September 2024.  References and Resources Further reading Harnessing homologous...

...organizers. My overall impression from this conference was that Cancer Researchers and Immunologists...Here I aim to compile a few highlights from the conference which reflect how cancer/immunology researchers...multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting presentation by...our Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed...about our free deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction...

...developers work in a streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with... part of my job is getting to talk to so many different people! I communicate with customers, software...Often, product managers will have three or more concurrent projects in varying stages, which can get messy...meetings. Meet with my boss and coworkers, discuss current and future features being built for the analysis...blogger Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore...

... a screening strategy as well. In a screen, you aren’t killing off a portion of the cells like in a selection...You’ll want to pick enzymes that will give you different sized bands depending on whether the plasmid contains...using vector-specific primers, look for size differences. You can also use primers where one primer anneals...popular sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of... Sanger sequencing Read the Molecular Biology Reference ...

...commonly used in research laboratories to detect the different subclasses, but there are also secondary antibodies...secondary antibodies to detect multiple targets with different mAbs in the same experiment. The Trimmer Lab found...within the plasmid backbone to generate R-mAbs of different subclasses, including subclasses that were not... useful research materials and information. References and Resources Additional Resources on the Addgene...Addgene's recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT...

...the wrong everything else.      References and Resources References Boston University Flow Core. Fluorochromes...standard curve, another quick experiment, or using a different vial and saving the poor, abandoned antibody for...assays involve multiple antibodies, each with a different signaling molecule, that all need to be detected...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...