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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE;

Showing: 141 - 160 of 610 results

  1. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses...ideal behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting...hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test...

  2. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... to you: our first, second, and third place winners!  1st Place: Krampus Saves Christmas! created by the... Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team...Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by Joanne Jary from Lab ...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification was impossible...light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics As with many TALEN-based... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...cultures because you are not the first person to face this kind of problem. Educate others when you can...

  5. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping... 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases...you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica...

  6. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...or by project, if that works better for you) and place them on an easy to reach shelf. Make sure all notebooks... Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the notebooks... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...find to where the binder was moved. You’ll have peace of mind knowing the information is backed up and...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more... structure to interrogate the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the...perfect guide RNA will do you no good if there is no adjacent PAM to anchor it. However, Cas9 only checks the...HNH domain, ensuring all binding partners are in place before cleavage can occur (Jiang & Doudna, 2017;...

  8. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity... clones because the bands were all in the wrong place. Except for one. My manager speculated that the ...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...best place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule...calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking...

  10. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...special words about managing scientists because, let’s face it, we are special. Below are some special characteristics...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...scientists) Are highly mobile and can move to a new workplace if opportunities for learning and personal growth...

  11. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...science writing in magazines or newspapers or other places online, and consider how those stories are being...their blogs. 5)   Local science museums are a great place to find opportunities for science outreach. Here...institution; the news or communications office is a good place to start. It’s not unusual to find science writers...Mass Media Fellowship or maybe an internship at a place like Science News. Ask your institutional news office...

  12. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genes. These data highlight an interesting "arms race" between phages and prokaryotes; the ultimate victor...

  13. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...some marketing experience, with little emphasis placed on the marketing or business background of the ...focus on the list of conferences I presented at and placed them under headlines entitled “Presentation Experience...more than necessary for my interview. The simplest place I could think of to start was “Marketing for Dummies...outside of academia, sales and marketing can be great places to start. Depending on what type of career you ...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...originally published in Nature, is a “search-and-replace” genome editing technique that mediates targeted... efficient due to its lower dependence on PAM placement. Fewer byproducts and more efficient than homology-directed... formation slightly. Designing the sgRNA with a spacer that only binds the edited strand, as in the PE3b... & Liu, D. R. (2022). Programmable deletion, replacement, integration and inversion of large DNA sequences..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  15. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...remember that industry career paths are not the only place to develop “industry related skills”. Many career...self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert skills...Google Scholar account Sanitize your LinkedIn, Facebook profile, online presence (if you need to).  Immigration...for international candidates. Being able to “put a face to a name” will help a candidate get looked at and...investment banking and venture capital areas.  We place individuals into roles in areas including: research...

  16. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...both conferences, there was a Q&A section in the interface where you can type in questions for the speaker...posters could be viewed publicly and stored in one place if the participants used the conference hashtag ... says.Depending on the meeting, there may be a “place” for networking within the app. For example, the... an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you can...

  17. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...and novelty in scientific research and in any workspace. Re-design lab meetings with a focus on discussion...making lab meetings more interactive and creating space for discussion at the end of each talk - time for...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ... purposeful and creative collaboration in the workplace. There are many other ways to encourage collaborations...

  18. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,... visit our website and follow us on twitter or facebook. If you are local, join us for our next event ...

  19. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...Simultaneous fluorescence (upper trace) and electrophysiological recordings (lower trace) using OCaMP expressed ...expressed in mouse cortex. Bottom: Zoomed-in view of traces corresponding to the dashed box above. The number ...potential (AP) spiking events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...example the Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...
Showing: 141 - 160 of 610 results