...production system may depend on what downstream applications you are planning, cost, and the amount of protein... for this. Chromatography columns containing appropriate immobilized ligands are incubated with cell culture...Quantification Once you’ve collected your antibody in the appropriate buffer, it’s time to quantify it. There are several...accurate concentration to give your downstream applications the best chance at being successful.  Using ...they bind their antigens specifically. Possible applications for validation include western blot, immunohistochemistry...some antibodies are better suited for certain applications. An antibody that performs well in immunohistochemistry...use! You can learn more about common antibody applications in our Antibodies 101 blog series.  Resources...

...to take a different approach. His own most impactful learning experiences happened in the lab, and he ...experiences afforded to those fortunate enough to apprentice with top scientists to larger groups of trainees...notes the importance of acknowledging student apprehensions about “failure” (protocols not working, hypotheses...while reminding participants of the value of this approach and encouraging them to engage in the problem-...in setting up a CURE, especially if it’s a new approach within their department. So in 2016, Provost partnered...research-based laboratory – a semester-long research approach using malate dehydrogenase as a research model...

...many CRISPR applications, and they’ve also had success in clinical gene therapy applications. Read on to...to learn more about the current (and future) applications of lentiviral vectors! Lentiviral vector history...barcoding libraries have been used for complex applications like lineage tracing in heterogeneous tumors...developed integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a...treatment of a molecular disorder: gene therapy approaches to sickle cell disease. Hoban MD, Orkin SH, Bauer...Integrase-defective lentiviral vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb...

...study for late career scientists to become more appealing on the job market (e.g. a postgraduate degree ...so much better. Driven by that, I successfully applied and worked as a technical support specialist for...expertise in technical support positions. When applying for technical support positions, you should try...company. To increase your chances at successfully applying for a position, you should familiarize yourself...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...different backgrounds and realizing that the same approach does not work for everyone. The benefit is that...

...use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town... makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a: An RNA cleaving...identity to the spacer sequence and contain the appropriate PAM sequence. The non-specific cleavage is thought...discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using Cas13a as...of nucleotides.   What are the potential applications of Cas13a given what we know about its structure...CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was originally published...development seen in the field and as always, we appreciate the opportunity to share these invaluable reagents...

...requiring people who are willing to quickly learn, and apply their knowledge to a variety of problems. In this...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients manage...Personally, I have greatly benefited from and appreciated the training I’ve received in concisely and accurately...literature, and critical analyses skills were applicable to my cases. I can directly use my understanding...core of the suite of benefits a given therapeutic approach brings to patients, doctors, and insurance providers...the gap between the science and the commercial applications of a particular therapy. With your scientific... research and bench science, with a newfound appreciation for how technologies get developed and commercialized...

...may be found at very different levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression...is codon optimization? Codon optimization is an approach in gene engineering to improve gene expression...sites and even affect protein function (Mauro and Chappell, 2014). Different rates of translation by different...and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is important to take a ...//doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-...https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

...very little data on which might work in your application. It can be quite difficult to decide on which...be searched and sorted by gene/target, name, application, pass/fail rating, sample species, antibody species... how the antibody performed in the submitted application, along with a brief description of the experiment... section will vary slightly depending on the application — flow cytometry, western blot, immunohistochemistry...data showing how the antibody worked in your application. The submission process is easy, with simple,...simple, descriptive fields, drop-down boxes when appropriate, and can be saved at any point throughout the ...

...robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat Chem...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...phytochrome (BphPs) photoreceptors have higher apparent cellular brightness than previously reported widely...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

... Brainbow 3.2. Brainbow labeling has also been applied to cell marking and lineage tracing in other systems...Between Brainbow Systems   Brainbow variants and applications beyond the mouse brain In addition to improving... Currently, Brainbow techniques are also being applied to study model organisms such as Drosophila and...zebrafish. Brainbow in Drosophila has aided in the mapping of neural circuits, such as connections between...Brainbow’s colorful labeling has decreased the mapping time for a given section of brain by at least an...brain and other biological systems. Interested in applying Brainbow to your research? Check out Brainbow ... Nov 1;450(7166):56-62. PubMed. A technicolour approach to the connectome. Lichtman JW, Livet J, Sanes...

...New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping...lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways to further...but what about all the manufacturing steps that happen before you even receive the product? How can we...tips. This is part of a cultural change in how we approach waste that needs to be supported at all levels... facility, where it’s sanitized through an EPA-approved process and no longer considered hazardous. Then...industrial and residential markets. Rethinking our approach to lab waste is something we all need to do. Remember...

...improvements to CRISPR technology since its initial applications for genome engineering in 2012.  Many of these...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed...allowing DNA binding and cleavage. This technique was applied to create photoswitchable spCas9, dCas9, and S....saCas9) proteins, showing the versatility of this approach. This study generated the first optically active..., allowing the release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated...Deiters’ lab, on the other hand, took a different approach. These researchers used a genetically encoded ...Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced Dimerization ...

...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry...space. For many cell lines and tissue types, appropriate reference genes (and supporting data) are often...2002), though there are cases in which it is an appropriate choice (Panina, 2018). Look for studies specifically...Vandesompele, 2002) will help you select the appropriate reference genes - and it is well worth your time... more, take a look at their 551 page e-book on applying MIQE guidelines! With these kinds of resources...melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April ...

...diagnostic applications. For example, fluorescently and radioactively labeled sdAbs have become appealing candidates...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative... as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical trials (...maintaining a llama facility is out of reach and unappealing to many labs), the field is moving towards using...inspired this versatile family of tools, the applications they have already enabled, and the discoveries...

...of the protein of interest. An electric current applied to the gel causes the negatively charged proteins...with blotting paper and an electric current is applied causing proteins to migrate out of the gel and ...sandwiches.   Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode...search, first pay attention to the validated applications listed on the vendor’s website and choose an...western and vice versa.  In addition to validated applications, pay close attention to the controls listed ...choose antibodies that are validated for your application and test the antibody in your specific system...to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences...

...attB-attP pairs from different recombinases are overlapping or nested on a register, then the operation of...more population-wide (as opposed to single-cell) approach is to interrogate state with qPCR. The re-arrangement...performed on one register at a time. If a particular application makes it difficult to isolate single registers...state read-out method may not be suitable for applications that require minimal cellular perturbation. ...contains the register. Depending on the desired application, the inducible promoters we used to drive recombinase...program to find the one that best suits your application. How efficiently do RSMs work? We tested a two-input...anticipate that, depending on the context and application, some inducible recombinase systems may work ...

...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...BE3, nicks the unmodified DNA strand so that it appears “newly synthesized” to the cell. Thus, the cell...reduce the undesired C→G or C→A conversions that can happen with BE3s. These byproducts likely resulted from...making them a promising tool for cell therapy applications. Dual base editors What about combining the ...Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion...activity compared to ABEmax. In an alternative approach, engineering and directed evolution of the TadA...editors with increased activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https:...

... be used as a fusion tag for a wide range of applications from traditional imaging to FRET and stochastic...transforming E. coli with a gene library of approximately 484 variants, including some potential tetramer-breaking...Florida State University, they used a mutagenesis approach and found that specific amino acid changes favored...violet range. For example, mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not...fluorescent protein: structural characterization and applications in fluorescence imaging." Biochemical Journal...identification of the molecular determinants of color and applications in fluorescence imaging." BMC biology 6.1 (2008...

... Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia... have been completed. Therefore, to be ready to apply for a Group Leader position, key first author manuscripts... project. Furthermore, Group Leader position applications require 3 letters of reference- the graduate...informal recommendations, more positive grant application and manuscript reviews, etc. You'll also meet... fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance runner, gardener...

...possible tools, lentiviruses for example, are not approved for use in vivo use at the University of Manitoba... be mostly on the brain, but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts... infect) can influence an AAV’s value greatly. Applying this mantra to AAV selection, there are three ...of AAVs? 1) Combined functional and anatomical mapping of circuits In our lab, we use AAV-DREADD complexes...only can we compare these methods in specific applications, we may also be able to use these comparisons... on overall cell function. AAVs are the most appropriate vehicle for this type of research, as they are...efficiency, and safety profiles should strengthen its appeal for the scientific and medical communities. Many...