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We narrowed to 330 results for: ARIA;

Showing: 141 - 160 of 330 results
  1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...
  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...
  3. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing...out this post. Learn how to Choose the Best Cas9 Variant for Your Next Experiment Read more about Optogenetic...
  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...
  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...
  6. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...
  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...–10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1–10) Improved split fluorescent proteins...
  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...
  9. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...unique plasmids does your library contain?), the variance of size of plasmids in your library (Are the inserts... Library Type Name Number of Plasmids Variable Region Size DNA per Amplification Amplification...
  10. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...stick out to form the “arms” holding the highly variable regions that bind to specific antigens. In contrast... Cloning System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press...
  11. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to...
  12. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...covered separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement...  Maria Genco...
  13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends...
  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...
  15. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...
Showing: 141 - 160 of 330 results