We narrowed to 567 results for: CHI
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TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....2017 contest winners: Chicagoland's Community Biology Lab (@ChiTownBio) and one of our favorite twitter...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...
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Podcast: Interview with Addgene's Founders
TypeBlog PostPublishedApril 13, 2018, 12:51 p.m....business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene... Director of Technology for 7 years, and is now Chief Software Engineer at Ginkgo Bioworks. He also holds... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog PostPublishedJune 15, 2016, 1:30 p.m....various cloning methods and tools to construct chimeric cellulases that enable E. coli and Citrobacter...plans for these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students...Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org Learn About... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m.... members Tony Cova and Chiara DeNatale and programming with our Chief Architect Pam McA' Nulty (check ... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD lab carefully...opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an imposing endeavor. Break...the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career topic that... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, ...likely that the phages are lost if they are not archived. “It’s very sad because it’s years of work,” Sacher... it can also fill this role by cataloging and archiving information on individual phages. Integrating ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading... Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be in close ... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m....in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m....turn up the heat when there is a bad Northeastern chill. Check out Joanne's Reddit AMA Few of us spend... in this country and we are quick to throw out chipped or imperfect equipment. We can’t safely recycle...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide...still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with...Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP GFP is more than a peptide –... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared to ...dCas9 fused to cytidine deaminases like APOBEC. To achieve this they created a lentiviral vector system that... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....donated to ABRC by the Arabidopsis research and teaching community through the TRAINED initiative (Translating...ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help setting ...professor? Whether you are looking to develop a new teaching resource or you want to introduce Arabidopsis ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....PMCID: PMC1209583. Watanabe, Tsutomu, Chizuko Ogata, and Sachiko Sato. “EPISOME-MEDIATED TRANSFER OF DRUG...and Esther M. Lederberg. “Sex Compatibility in Escherichia Coli.” Genetics 37.6 (1952): 720–730. PubMed ... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various... Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons. We very much appreciate feedback... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...PMID: 29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m.... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ...These new processes have been instrumental in achieving our mission to help science move faster in the...Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus...