Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 623 results for: Dos;

Showing: 141 - 160 of 623 results

  1. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...

  2. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...

  3. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...alone; a researcher doing some experiments in Brazil can have the same issues as one doing experiments in ...sometimes wish they could live differently than we do day to day. We discovered that it can be good for...

  4. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    Published
    Nov. 8, 2018, 3:47 p.m.
    ...“Synthetic biology, in particular, the ability to do DNA synthesis, is evolving so fast that it is a really...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... engineered? Where did it come from? What does it do? Those are the sets of questions one would want to...

  5. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument... science with the community. Some of them you can do today! 10 ways to share your science 1)   If you ...talk – really talk – about the research you are doing in the lab with your mother or father or your best...

  6. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...support your career development as your mentor. How do you make the most of this new relationship? Consider...skills in which you have expertise or knowledge. Where do these intersect? Work with resources: Choose books...take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...

  7. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should...the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The...

  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...the population that have an edit. I didn’t do this, but to do it you would need a pretty good gel image...Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure ...expansions (monoclonal, 1-5). Wild-type A549 cells do not express CAs9 and are included as a control (WT...couldn’t get a good image. You could probably also do Western blotting against GFP expression, but I didn...designing primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...are the perfect person to be writing the review! Doing so is a great way to develop your ability to write...here, I will share my process with you. But before I do that, let me say that I was by no means an expert...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write ...article and that someone is going to be you. So, just do it! I will also paraphrase what I have heard many...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...Satellite colonies are small colonies of bacteria which do not contain the plasmid and its associated resistance...effectively selecting plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative...Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin is handy because ...above antibiotics would work for you, they likely do! Give our summary table a look for a quick recap ...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...explaining how your experience and skills would help you do the job as described. Let’s imagine you’re applying...example, if you don’t have experience with ddPCR, but do have experience with qPCR, you could talk about how...that are excited about their work and mission.  How do I know what to write about? If you’re struggling ...that skill. If the answer is yes, ask yourself “how do I know that I have that skill?” The answer to that...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...people develop the disease while others never do, but we do know that there are some genetic factors involved...much of a danger of getting the disease, even if you do happen to be exposed to asbestos. However, as the...cancer is diagnosed so late that these treatments do not work very well. The best option in such instances... mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, ...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... why you might enjoy doing it. I’ll also talk about the skills you’ll need to do well as a healthcare ...healthcare consultant and what you could do to break into the field. What is healthcare consulting? Healthcare...pulling your weight with your scientific expertise. Do I have to stick with consulting in the long-term?...and services that help diabetic patients manage doses and timings of their drug treatments. The process...

  15. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...the situation in the future. Addgene: What advice do you give to post-docs and graduate students as they...publications in a short period of time and may need to do some fashionable projects. However, I hope that they... they also have long-term goals and continue to do research to realize their vision. Their progress might...they'll be completely different from what we are now doing in my lab then when they become independent. Looking...

  16. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...same transcript. Of course multicistronic vectors do not have to exclusively be used as a means of detection...levels between two or more genes is important. How do multicistronic vectors work? Scientists have "borrowed...commonly found in bicistronic vectors; however, they do have some disadvantages. These elements are quite...of the peptide to improve cleavage efficiency. How do I get started? If you are looking to co-express your...

  18. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...collection (what science has been hot this year and how do you creatively turn it into an outfit?), and of course... box. Which is your favorite team costume so far? Do you have any suggestions for this year’s teams that...

  19. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...detection So you found mycoplasma contamination, what do you do next? The best way is to discard the infected ...contamination before they enter your cell culture and what to do if you encounter mycoplasma contamination. Causes... optical microscope can be very difficult, so how do you know it’s there? For this task, you can use PCR...before you even realize it. Here are some things to do: Wear a clean lab coat and mask while handling cell...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...enable efficient base editing and prime editing. How do you then set up an MBE system that contains a double-win...editing, while keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism,...exhibited reduced Cas9-dependent off-target editing and do not cause higher Cas9-independent off-target editing...
Showing: 141 - 160 of 623 results