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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 379 results for: ENA;

Showing: 141 - 160 of 379 results

  1. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban... Ban Asbestos Now Act of 2016 in the U.S. Senate. If this bill passes, it will put an immediate stop to...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... courses of treatment with antibiotics These scenarios expose bacterial populations to antibiotics at...counter-intuitive considering the other three scenarios, but a short treatment regiment might wipe out...

  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...cells (see Figure 1). When behaving properly, FPs enable researchers to investigate the localization and...undeniably powerful and successful tools that have enabled numerous cell biology assays. However, there has...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Proper controls provide the constant variables that enable the correct interpretation of the effect of the...transfection control measures transfection efficiency and enables observation of any effects of the transfection ...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...The modular system of linkers and spacers could enable many future variations using nanobodies or scFvs...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). ...

  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ... embryonic development, extended-open integrins enable tissue morphogenesis, organ development, and the...integrins demanded higher tensions before they would enable cells to spread, resulting in cells that elongated...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...demonstrated excellent counter-selection which enabled very efficient recombineering.     We were inspired...CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... or drug selection, assuming your reagents are amenable to this. If delivery isn’t an issue, you may need...M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul...

  12. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It...multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even be battery operated,...actively interested in science communication and enabling access to science in developing countries. You...

  17. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...ELISA and cell surface display are common. ELISA enables quantitative measurement of antigen-antibody interactions...mutant libraries on the surface of cells. This enables direct screening via flow cytometry for improved...

  19. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... al., 2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand...one self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all genes be ...antibodies for immunocapture applications and is amenable to high-throughput analyses, however, it requires...
Showing: 141 - 160 of 379 results