We narrowed to 180 results for: ENDO 2

...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...that tests enhancer sequences outside of their endogenous chromatin environment and relies on the fact ...because it was difficult to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports...genes of interest to generate mAID fusions to endogenous genes. Cell lines with the appropriate fusions...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating... using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor ...naturally occurring cancer-causing mutations into the endogenous allele of the STAG2 tumor suppressor in human...gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant...dominant mutations of a gene into the endogenous allele in cultured cells. AAV genome editing combined with ... 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes in diverse human cell lines." Nucleic acids...

...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...the complex is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily... DNA strand, and then activate two different endonuclease active sites. Fortunately, there is never just...

...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to...Additional libraries are also available from commercial vendors. These libraries usually contain 4-12 gRNAs/gene...

...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...optigenetically and chemically excited a class of enteroendocrine cells within the intestines known as neuropods...

...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed...

...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...The choice is non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...

...Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, ...signaling can also modify other properties such as endogenous gene expression, which can lead to changes in...with the goal of creating receptors with less endogenous activation and reduced off target activation ...

...promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your antibody...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In.... In two studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually...

...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...2009). However, these reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they...

...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...Tailored mutations are commonly introduced to endogeneous DNA through homology-directed repair (HDR) of...editing requires a template of high homology to the endogenous target, yet to facilitate the repair, the template...template is removed using a methylation-dependent endonuclease (i.e. DpnI), and bacteria are transformed with...

...retroviral library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express ...allows for whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc...

... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic...

...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...cinch. This is because it doesn’t secrete its own endogenous proteins, making the recombinant protein its ...

...responsible for that differentiation.   Fig. 2: Single cell RNA-seq clustering of cells transduced...cells resembling all three germ layers (ectoderm, endoderm, and mesoderm). These profiles were then compared...

...inhibit editing (Anzalone et al., 2019). Figure 2: Starting lengths for optimizing your pegRNA primer...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs...

...and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In...the IP landscape, using plant parts which are endogenous to the plant, off patent, or not patented, and...

...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells...