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Showing: 141 - 160 of 520 results

  1. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...reagents) along with information critical for the performance of a reagent, e.g., antigen retrieval conditions...Knowledge-Base is an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX ...

  2. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

  3. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

  4. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero...

  5. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ... or drug treatment can browse through screens performed in relevant cell lines or conditions. This increased...searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...tracrRNA sequences in a “single-guide RNA” that performs both functions. Cas9 cuts both the target and ...RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between the guide...

  7. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

  8. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening...The diversity of the CRISPR/Cas systems provides powerful lines of defense against invading phages. Rising...

  11. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...beta-galactosidase cassette allowing a researcher to perform blue-white screening after transformation - self...7798275. Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis...

  12. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for... very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain samples. For large brain...

  13. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ...

  14. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...your choice. First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus...still express episomally long-term (AAV). If you perform a lipid-based or electrical-based transfection,...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...system for multidirectional control The CRISPR interference (CRISPRi) model consists of a catalytically ...field of metabolic engineering, this is such a powerful and flexible technique that it should be on the...

  17. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example, Dave Grawoig...Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments for discovering novel...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...the gradual change in pH visible at the sample interface upon thaw (Fig. 2). Before thawing, all the samples...study found that dry ice storage affected the performance of antibodies, because of effects of pH on protein...

  19. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...lentiviral vectors from the repository for you. We perform test infections with all of our viral preps and.... We provide enough lentivirus for you to both perform an optimization-scale infection and at least one...
Showing: 141 - 160 of 520 results