Skip to main content
Addgene
Showing: 141 - 160 of 282 results

  1. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... with soluble fluorescent proteins or localized fusion proteins and do not require dedicated plasmids ...performed on purified fluorescent proteins. To avoid diffusion, the proteins are (i) trapped in microdroplets...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...orthologs NmCas9 and St1Cas9. Each ortholog was fused to a different fluorescent protein to create three... recruit fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs ...

  3. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...   Prior to the polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks... to scientists. If their protein of interest is fused to polyhistidine, then they can use an anti-HIS ...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...I suspected I knew at least one source of the confusion in our meetings. A lot of our LIMS planning involved...can help the team identify possible points of confusion in the future. Improving cross team communication...

  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... as clear as you can be (there will always be confusion about this though) about the fact that containers...them to their contents might leave you sad and confused when a tricky procedure failed and one of your...

  8. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments. You ...windows” you’d like the tool to use to identify fusion sequences for either a circular or linear assembly...

  11. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...varied as it can have important implications for the fusion protein effectivity), and a fluorescent protein...degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible...

  12. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...(SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct...flexible. Like the xCas9 3.7 nuclease, xCas9 3.7 fused to third generation cytidine base editor architecture...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system...in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but...

  15. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...across a couple other terms that can cause some confusion. Paraformaldehyde: Many IF protocols call for...crossing cell membranes as they are not able to diffuse across natively. Permeabilization essentially punches...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...swapping and generating N and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, ...Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants’, Bioscience...

  17. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...construct, 3 copies of the fluorescent protein were fused in-frame to a tethering partner, which directed ...enzyme choice is critical. May not support in-frame fusions of coding sequences Sladitschek and Neveu, 2015...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ... 18;127(7):839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene ...Cavazzana-Calvo M, Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of...antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright targeted...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor...
Showing: 141 - 160 of 282 results