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We narrowed to 450 results for: HA tag

Showing: 141 - 160 of 450 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...enormously plastic tool and has taken the scientific world by storm. While Cas9 has been most widely used to..., Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression... to create specific edits in DNA, there has also been significant work on constructing Cas9 transcriptional...CRISPR activation for genes with known functions has the potential to be used in therapeutics, as it can...changes in phenotypes. For example, CRISPR activation has been used to differentiate induced pluripotent stem...creation of innovative technologies. To date his group has develop several popular CRISPR-based tools for altering...Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find...

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...community has always emphasized the need for open science and collaboration. The field already has comprehensive...system a modified TIR-1 protein can degrade degron-tagged proteins in an auxin dependent manner. This system...first virtual organism in a computer.” This project has produced various resources for the worm community...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...screening, Single-cell Phenotypic Observation and Tagging with Light (SPOTlight) as a way to isolate individual...Cas12a. This is in part due to the fact that it only has a single active site. The RuvC active site in Casɸ...rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ...library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency and reduced...

  4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...dye, but has been modified with the addition of two iodine residues. Compared to MG, MG-2I has increased...development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization and expressed...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...and mammalian. These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...controlled setting. Read more: Plasmids 101: Protein Tags Produce proteins that glow so that scientists ...be used in research or for therapeutics Addgene has compiled various educational resources to facilitate...plasmid) and typically include a gene that is advantageous for survival, such as an antibiotic resistance...

  7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...looked hard at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion... for the CZ Biohub project by Manuel Leonetti to tag all 22,000 protein coding genes in the human genome...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in the Biohub.... tools which were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide...cut-to-insert distance for HDR. (The notable exception is TagIn––see their "summarisation score".) For CrispyCrunch...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have good ...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of press. However, this process accounts for a percentage of the mutations seen with TALENs and CRISPR....follows. For increased versatility, it would be advantageous to adapt PITCh to insert genes into AAVS1, the...using the nuclease Cpf1. Since Cpf1 cuts in a staggered pattern, it is thought to be ideal for HDR-independent...and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...also the fastest test that has been developed so far.  A similar method has been optimized to detect the...Two-step-SHERLOCK has a sensitivity in the zeptomolar range while One-step-SHERLOCK has a sensitivity in...using lateral flow strips. Since the probes are tagged with streptavidin, the lateral flow strip first...State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting...full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around the...the outbreak ultimately flattening the curve, it has become evident that widespread testing is crucial... 83 known COVID-positive samples tested, DETECTR has a positive prediction rate of 95% and a 100% negative...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...to allow in-frame deletions and introduce epitope tags and RNA transcription start sites. The lab further...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization...fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) ...Markus, and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...an FP-chimeric protein may be at any one of many stages of synthesis and degradation. For this reason it...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't...

  12. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...until the pups are 2-4 weeks old and use the ear tag method (creating different patterns of ear holes ...Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded since its adaptation for use in mammalian...biology surrounding a particular gene (although it has been quite successfully adapted for use in screening... method for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for...sturdier than the delicate RNA and therefore there has less chance of contamination with RNase leading to...products will give specific details on what exactly has been inserted or deleted. Each lab will use a slightly... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... applications (v82 has higher affinity for glutamate but slower kinetics; v857 has faster kinetics but...but lower affinity) and with a variety of tags for localization (PDGFR, GPI, and SGZ). Read more from the...iGluSnFR3 (Aggarwal et al. 2022). This new version has higher signal to noise ratio and is better localized...for two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness...and deep-tissue imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium indicator...interest, it’s important to choose a biosensor that has a binding affinity close to the range of concentrations...physiology and metabolism under various conditions. LiLac has improved response size and reduced sensitivity to...

  14. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...epitope-targeting antibody that is conjugated to a biotin tag but not a fluorophore. In a separate step, cells ...interest. Indirect labeling is achieved through protein-tag or protein-protein interactions, like biotin-streptavidin...selecting the primary antibody host species, as it has to be different from the other antibodies in your...target marker, allowing for its detection. The advantage of this system is that streptavidin conjugates...the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility for panel...

  15. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from...the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that (in the... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...activator (1a) or a repressor (1b). Scientists took advantage of these discoveries to create a new system for...subset based on a cellular compartment or growth stage). A prey library can either be applied to the bait...

  17. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...the the developing zebrafish pancreas. They first tagged these cells using plasmids. Then using HOTcre, ...more commonly known: the fruit fly. The fruit fly has been used in scientific research for over a century... a transgenic mouse line. Over the years the fly has become an ideal model organism to study an array ...development, genetics, and the nervous system. The fly has also emerged as an important human disease model ... described in 1993 by Norbert Perrimon’s lab and has been continuously improved upon since then. If you...Zebrafish (Danio rerio) Since the 1960s, the zebrafish has gradually become an important model organism. They...Caenorhabditis elegans) The worm, Caenorhabditis elegans, has been used extensively as a model organism since the...

  18. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection... As I wrote in the blog post, BUILD YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based...

  19. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...any real traction.  This can carry a heavy price tag, especially if you're working on something other ...this deter your ambition. If you believe your idea has potential to make a difference, then make sure you...Boston-based startup focused on empowering early-stage scientists. You can think of miLab as a sort of ...biotechnology investment fund that would apply a percentage of returns to support student projects, but it...for such a fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback...against you.  As I mentioned, you start at a disadvantage in the health science space if you don’t have...

  20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ... cloning procedure adds both V5 and His6 epitope tags to the C-termini of Met proteins, which can be used...relatively confident that the gene from the test species has a similar function to the gene in the reference species...left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell biology...
Showing: 141 - 160 of 450 results