We narrowed to 161 results for: Kars
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TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination of HIV-1 Genomes...
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Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....2010) and the MAGIC Marker strategy described in Karine Loulier, et al. (2014). Currently, Brainbow techniques... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....let’s take a moment to recognize your little prokaryotic minions that carried out the labor-intensive ... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber, T. L. (2021... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....dCas9 to a small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m.... it has been repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....Hopkins University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... 3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional insights... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....number. RNA interference (RNAi) is a conserved eukaryotic process where approximately 20-30 nucleotides... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....mycoplasma enzymes that are not synthesized by eukaryotic cells. First, the kit components rupture the ... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....Protein expression in yeast MoClo is also for eukaryotes! The technology has been used to optimize protein... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....:18658221. PubMed Central PMCID:PMC2527278. 9.Karbowski, Mariusz, et al. "Quantitation of mitochondrial... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m....): 517-528. PubMed PMID: 25319501. 7. Palucka, Karolina, and Jacques Banchereau. "Cancer immunotherapy... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....bacteria, a few manufacturers (such as Zymo and TakaraBio) offer yeast-plasmid specific kits. Medium/High-throughput... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....polycistronic glycine tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, ... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m....Minicircles are like mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and ... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....2016). Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....Hantman, A.W., Looger, L.L., Schaffer, D.V., and Karpova, A.Y. (2016). A designer AAV variant permits efficient...