We narrowed to 200 results for: LRE

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...working in plants, including many things people already use. It makes the switch to Golden Gate Cloning...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...engineering. What's incredible is the bioeconomy is already pretty substantial. It's estimated that genetic...

3. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ...the 8th grade science teachers socially and we’d already discussed the idea of my giving a CRISPR presentation...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ... loci. Many applications of these systems have already been demonstrated, ranging from large genomic insertions...

5. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ... and suggestions are not so much inaccurate as already somewhat dated. Zimmer could not have predicted...

6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...rolling mean. While this global approximation already shows us a rough "COVID-19 effect" on order data...

7. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...particular scientific question - even when others have already answered this question and used it to publish!  ...

8. Getting to Know Expansion Microscopy

   Type

   Blog Post

   Published

   June 20, 2023, 1:15 p.m.

   ...inexpensive reagents and are readily adoptable by labs already familiar with IHC/ICC protocols. A typical ExM ...

9. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...is to utilize the built-in repair pathway cells already have for repairing DNA double strand breaks – (...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ... types of bacteria, such as SHuffle cells, are already resistant to this antibiotic due to stable integration...

11. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ... be beginning to realize that the situation is already complex enough that a deep understanding of retroviral...

12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... an experiment, and want to see if someone has already created and deposited it with Addgene. Combining...

13. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...expected size, you know the genome of your bacteria already contains your target sequence. Additional Resources...

14. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ... cannot be used in eukaryotes because there is already too much SUMO protease around, but it is convenient...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...Advantages Disadvantages Direct Fast and simple protocolReduced chance of cross-reactivity Higher level of...

16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ...beauty of embryo development in real time. I was already a biology geek, but this formative experience nailed...

17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    Published

    Nov. 19, 2020, 2:15 p.m.

    ...Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878 Sinnett SE, Hector RD, Gadalla KKE...

18. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ... no need to codon optimize as this gene should already be biased to that organism's codon usage. Others...

19. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...dependent on the cell’s intrinsic machinery being already activated to work. (It was also found to be independent...

20. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...appropriate flanking restriction sites and overhangs already added. This way your synthesized DNA fragment can...