We narrowed to 217 results for: LUM

1. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...

2. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ... lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology, the Dept. ...

3. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...colonies (5-10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic...

4. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   Published

   June 27, 2019, 12:25 p.m.

   ...natural environment. Thus, the Harris Wang lab at Columbia University developed a method for genetically ...

5. Celebrating 15 Years of Scientific Sharing

   Type

   Blog Post

   Published

   Jan. 14, 2019, 2:08 p.m.

   ...the Addgenies and congratulations to current and alumni Addgenies on our success. Thanks to our amazing...

6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   Published

   Nov. 3, 2020, 2:15 p.m.

   ...confines channelrhodopsin activation to a small volume of brain tissue (~1 mm3). Current channelrhodopsins...

7. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...labs are quite happy to share, labs that get high volumes of plasmid requests can end up refusing such requests...

8. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...easy detection with the appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments...

9. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ... a bunch of random solutions when all the spin columns run out. In this article, we cover the basics of...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been...

11. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...sequencing service will have different requirements for volume and concentration, and not all providers are equipped...

12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for...

13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ... to increased pressure of the added liquid. The volume should be minimal in order to minimize the pressure...

14. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole brains...

15. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...Successfully infected cells will become round and clump together ~3 days post infection. The amplified virus...

16. Rabies and Neuronal Tracing

    Type

    Blog Post

    Published

    May 29, 2018, 1:51 p.m.

    ...in biology is that the body’s architecture and plumbing are important. We started with learning the head...

17. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...research course that is part of the biology core curriculum at Boston College. Students in the course work...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...the group was able to demonstrate that while illumination of neighboring cells evokes action potentials...

19. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...pipette with how much total sample you need and the volume of the smaller aliquots you’d like to dispense ...

20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-...