We narrowed to 213 results for: LUM;
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TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....colonies (5-10) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic...
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Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....natural environment. Thus, the Harris Wang lab at Columbia University developed a method for genetically ... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....the Addgenies and congratulations to current and alumni Addgenies on our success. Thanks to our amazing... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....easy detection with the appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m.... a bunch of random solutions when all the spin columns run out. In this article, we cover the basics of... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....sequencing service will have different requirements for volume and concentration, and not all providers are equipped... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....mitochondrial membranes imaged with structured illumination microscopy in HeLa cells expressing COX8a-mStayGold... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....pipette with how much total sample you need and the volume of the smaller aliquots you’d like to dispense ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m.... to increased pressure of the added liquid. The volume should be minimal in order to minimize the pressure... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....Successfully infected cells will become round and clump together ~3 days post infection. The amplified virus... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....in biology is that the body’s architecture and plumbing are important. We started with learning the head... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....research course that is part of the biology core curriculum at Boston College. Students in the course work... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....the group was able to demonstrate that while illumination of neighboring cells evokes action potentials... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole brains... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m.... Wu, K., Wu, R., Xiang, L., Fang, X., Jin, Z., Goldblum, D., Hasler, P.W., Scholl, H.P., Krol, J., & Roska... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....Additional considerations for titering lentivirus Volume and transduction time will influence lentiviral...