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Showing: 141 - 160 of 398 results

  1. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...overtime and weekends, part time or full time) and what life is like for someone who currently in the position...online job websites to see what requirements or qualifications are needed for the role, as this will give ...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

  3. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...students focus on the application process, and simplify access to academic resources, we created STEAMid...Chemical Biology Program at Harvard. Roo wants to simplify access to academic resources among economically...

  7. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ... by using primers that are vector specific but amplify a potential insert. If you’re using insert-specific...no insert in the plasmid, you won’t be able to amplify anything. Like any experiment, you’ll want the ...

  8. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... Plasmids here! Xiong, et al. eLife. 2021. https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...

  10. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...Conventional thermal cycling is then performed to amplify the target using PCR. Instead of tracking the reaction...assays include a forward and reverse primer to amplify the target locus, as well as a reference probe ...

  11. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...is not necessary for the proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693...

  12. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...the scientific method to everyday problems in your life. This tactic is a good way to see how transferable...resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset...

  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

  15. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... Biology in a position working with preprints in life sciences. He also posted two preprints of his own...proposal for the future of scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...secondary antibody is ideal as it will help to amplify the signal. Some applications use either direct...that a secondary should be used when signal amplification is needed.  Species  Next, check the species...

  17. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ... and all 6,000+ postdocs at the University of California, who had a contract - negotiated by their union...salaries. Postdocs at the Salk Institute in La Jolla California are not getting a raise. Meanwhile, in less densely...

  18. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... your lab bench, we need to (this is a little simplified): Find the appropriate glycerol stock within...happy lab staff. Barcoded materials make everyone's life easier, the time and capital cost of getting up ...

  19. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...drugs were withdrawn from the US market because of life-threatening health effects. Eight of these posed...breast cancers, and 145,000 more quality-adjusted life years. While most of the results were positive, ...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our Sanger sequencing...number of sequencing reactions, you can make your life much easier by selecting the right primer. This ...
Showing: 141 - 160 of 398 results