We narrowed to 714 results for: MAK

...organism of choice. Unfortunately, codon preferences make it so you cannot choose among the possible codons...therefore stall at inappropriate locations or fail to make it through the entire transcript resulting in the...properly. You might then think, “Okay, I’ll just make sure the abundances of the codons I choose for the...surrounding codon choice, you should have some method to make sure the proteins you produce function properly....choice, you should perform a functional assay to make sure your protein isn’t forming inclusion bodies...

...structure and function of Cas13a with a focus on what makes this molecule unique, and the various applications...nuclease Cas13a was originally identified in 2015 (Shmakov et al., 2015). They were using Cas1, a gene commonly... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...excited to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture... Find plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional characterization...

...updated in several years) or they make significant changes that make it incompatible with our software...a problem, but it is these very differences that make our software so great. If we all came from similar...a bag of flour is not the only thing you need to make a cake. The flavor, shape, and texture of a cake...understanding the hows and whys of our users, we are able to make informed software design choices, resulting in software...

...exclusive access to data and enjoy reasonable time to make sense of it, with their decision to publish setting...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ...raised by researchers worried about the effort to make the underlying data easily available, organized ... the scientific community is thus not averse to making data available, but there remains concern about...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...

...through some of the most important considerations to make when planning and performing an IF experiment. ...antibodies in a few different species and colors makes it easy to perform multiplexed IF. By using multiple...Some of the most important considerations are to make sure your secondary 1) will recognize the species...Database and CiteAb. The antibody you choose can make or break your experiment, so take advantage of all...your experience, positive or negative, will help make antibodies more effective and reliable in the future...

...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently... plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder... than commercial ladders. Are you interested in making your own DNA ladder? You can find the Tan Lab’s...Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel...

...calculator can do the math for you and it’s simple to make adjustments for the type of tissue culture dish ...dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download the... spreadsheet: We hope that this calculator will make it easier to plan your AAV packaging experiments...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

...available the potential mentor is. If the person makes time for you, as needed, this is a strong indicator...time and resources. You typically have 4-5 years to make discoveries and publish papers to position yourself...most exciting story I can tell with my data?  Can I make better slides that more effectively tell a story...present your research. Face to face meetings can make favorable impressions on potential manuscript or...minimal processing, and low in empty calories will make you feel better and help maintain your health. ...

...twenty graduate student volunteers worked hard to make this Saturday event a success, a true testament ...communication gap between scientists and the public and help make science more transparent. Second, SITN helps graduate...intelligible scientific resources. SITN hopes to make DayCon a yearly event and to expand the conference...is a great idea and a group of people willing to make that idea a reality! How do you share your science...

...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest..., and fusion location, there are many choices to make. 2. Prime Editing: Adding Precision and Flexibility...

..., I’m picking two posts that describe methods to make single base edits in DNA. The first, contributed...a thorough walkthrough of how you can use PCR to make small sequence changes to plasmid DNA. This an oldie...CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by...informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis by PCR obsolete...

... a job you wish you knew more about – how do you make a connection? An Informational Interview is a great...relationship building through Informational Interviews. Making Your informational interviews productive Informational...phone or video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations...many good lists of questions on the internet. To make the most of the opportunity, use a written list ...

...Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references easy...for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine... tabs (for different experiments or projects) to make it easier to know where to start your search. Then...track the revision history. Now you’ll be able to make updates to protocols in real time. You may also ...

...contributed by our depositors, Addgene strives to make it easier for you to find the plasmids and publications...and the original depositor. Our citations feature makes it so that, when you cite a plasmid from Addgene...who may be working on similar projects and should make it easier for them to find your work later. We also...process. Special collections pages In an effort to make it easier for you to browse through the many plasmids...

...collection, the fluorescent proteins are, which makes mixing and matching a relatively easy proposition...Davidson lab for the last four years. “Researchers can make their own target if it is not already made for them...For more information on choosing a backbone and making fusions, see this blog post from Joachim Goedhart...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many...

... updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called...expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains...increases the chance that all the edits you want to make will happen.  There are many reasons to multiplex...constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors like... mammalian option described below, plasmids for making polycistronic gRNAs are also available from the...

... the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! X-ray crystallography...base. In Figure 5, we can see why: two arginines make base-specific hydrogen bonds to the guanines, but.... Residues R1333 and R1335 from the CTD (yellow) make base-specific hydrogen bonds to guanines of the ...The HNH domain, which cuts the target DNA strand, makes few contacts with the rest of Cas9 and is highly...BioRender.com.   The exact conformational changes that make Cas9 such a valuable laboratory tool are still an...

...adopting a few meaningful changes in the lab could make a big difference in our global impact as scientists... environmental impact, but we can all do more to make research more environmentally friendly. Adopt one...Universities and companies have the potential to make a huge impact directly on waste, but also on culture...bring ideas to your workplace and you could end up making a huge impact on how waste is handled. A lot of...

...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate...also has a suite of molecular biology tools and can make plasmid maps. Free for academic users. Serial Cloner... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications... Addgene blog: Learn how the CRISPR software matchmaker can help you choose the best CRISPR software ...

...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture... understand the importance of using analytics to make decisions on content strategy. I really believe ...different best practices, create an email strategy, and make the occasional TikTok. I was also responsible for... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...