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We narrowed to 469 results for: NOL

Showing: 141 - 160 of 469 results

  1. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ..., which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These variations directly...toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands Throughout the history...capabilities of fluorescence-based techniques in biotechnology. Recent examples of advancements include the...

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    .... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... variety of fields including neuroscience and immunology. They can be used for qualitative and quantitative...Historical Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 ..., IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...the history of neuroscience research we have a technology that allows us to show causality in neural circuits...technique doesn’t have its limitations or that other technologies need to be discarded but clearly we are in an...and relatively cheap (compared to many other technologies and techniques in the sciences). But how exactly...

  5. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...honest — kits are quick, easy, and don’t involve phenol-chloroform.  The default option is to use the ...things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins. The most common method for checking...issues. For example, some kits reduce the risk of ethanol carry-over into the eluate, while other kits are...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol. Although the...targets, we usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions....1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal perm/fix solution depended...

  7. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...human endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics... sector are transferrable to industry such as technology transfer, communications, medical writing, compliance...Casseus Law. Driven by her own love of science and technology, Rachel is proud to assist companies and foreign...

  8. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...without staining; simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...

  13. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

  14. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...

  15. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...

  17. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ... and adopted by the Association of University Technology Managers (AUTM). This MTA provides many benefits...and the receiving institution. Therefore, many technology transfer officers are familiar with the UBMTA...

  20. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590...subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590...
Showing: 141 - 160 of 469 results