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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: Ncl

Showing: 141 - 160 of 628 results

  1. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...genome. Typically, it is the sequences between and including the LTRs that are integrated into the host genome...internal promoter. An internal promoter must be included as the wildtype LTR promoter is no longer present...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...classes, some pick and choose a few lessons to include each year; others use BioBuilder as an afterschool... work with one of five BioBuilder kits — which include lab materials and reagents such as droppers, centrifuge...for both STEM teachers and STEM-loving students include The BioBuilder High School Apprenticeship, preparing...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this paper...editing rates vary widely from gRNA to gRNA, it’s unclear where this study would fall in terms of editing...

  7. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    .... In the case of stem cells, these factors may include cell type, the disease being treated, and other...immunological, and environmental factors. How to include sex in experimental design Recognizing the importance...Institutes of Health (NIH) now requires that sex be included as a biological variable in all NIH-funded research...

  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine steroids... to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...interface, expanded numbers of curated molecules, including 400 or so coregulators, and a greatly expanded...

  9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ... recognized by distinct proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic...local gene expression, confounding any potential conclusions about the role of methylation (Hrit et al. 2018... many effects independent of dCas9 targeting, including modifying other sites in the genome and directly...

  10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the quality of other services that we provide, including viral preps and pooled libraries. As anyone who...throughout the library preparation process and includes Picogreen quantification of various intermediate...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as CAR-T cells...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but ...recombinant antibodies as well? Our collection includes a mouse and a chimeric rabbit (original mouse ...

  12. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...paragraph and point out words or sentences that are unclear. We’re so used to conversing with other scientists...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

  13. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... of infection and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré...seven institutions involved in the effort. This inclusive and open approach by all involved definitely contributed...transformed the assay’s capabilities. Specifically this includes primer directed isothermal RNA amplification, ...

  14. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ... transferable into multiple bacterial species including several involved in human disease. However, the...every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer...Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic strains and environmental isolates....

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and ...detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials (...triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

  16. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... mice. While not an exhaustive list, these can include what’s being injected, the type of experiment, ...method. Many different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses.... the name suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...NgAgo optimization before we can make any major conclusions: That said, many people are having difficulty...Some great places to get your results out there include: Google Groups - I've specifically set up a forum...Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain cells...

  19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our model infers...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...

  20. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...mind that access and availability to resources (including the internet!) can vary depending on factors like...your best to understand what kind of resources - including your time - will be required to produce an outcome...naked eye or to make things more accessible by including, highlighting, or omitting details. Digital or...
Showing: 141 - 160 of 628 results