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Showing: 141 - 160 of 367 results

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...good, high quality preps due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability...these factors are generally, but not perfectly, correlated. Typical growth times for high copy plasmids...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...in mind! This section of our plasmid pages is relatively new, so some of our older plasmids may not have...here. Articles Citing this Plasmid: This is a relatively new feature of our plasmid pages, and provides...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

  5. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...SpCas9 (2). In many cases this is due both to lower relative nuclease efficiencies as well as to more stringent...tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs. It should...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy... our understanding of the structure-function relationship of the central nervous system under normal and...

  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...

  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...

  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...before then. Other areas are newer, such as the relatively recent resurgence of “Do-It-Yourself Science”...hadn’t made manipulating the genome cheap and relatively easy? Have all of these things contributed to...

  13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...

  14. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...

  16. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...reliably distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK...from cell-free DNA fragments as well as health-related single nucleotide polymorphisms (SNPs) from human...

  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10...edit in a stretched window ranging from 1-17 bp relative to the PAM sequence (Zong et al., 2018) (see CBE...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...
Showing: 141 - 160 of 367 results