We narrowed to 615 results for: REV;

...containing the desired edit adjoined to the sgRNA, a reverse transcriptase, and a nCas9. While this system has... Fig. 2: Prime editor engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand...successfully in experiments and published in peer reviewed journals. If you need to generate a new sgRNA ...your experiment. Keep an eye out for our upcoming review of these options in our CRISPR 101 series!  References...

...we give people the opportunity to speak out and prevent groupthink? Here are some ideas that I have found...a member of our Business Development team would review it. This survey generated 26 ideas, over half of...mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org... spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org...

...participant in the training. In this blog we will review the preparation and planning you can do to build...different way of doing something then you’ve done previously) you can bring those questions to your training...questions throughout training, while others prefer to review their notes, process what they’ve just learned,...Show up prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

...Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this...the expected sequence, the QC team can manually revisit the assembly in a program called Geneious. Within...the assembly. Geneious will show how reads align, reveal multiple contigs, and highlight sequence identities...mismatch or indel flags the sequence for manual review by one of our QC team members. This process saves...

...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...over-expressing a foreign protein in E. coli. We will review the potential pitfalls of recombinant protein expression... problem for toxic protein products, which can prevent the culture from reaching the desired density within...

...unlikely to be found in your gene of interest) and prevent incorporation of extensive sequences used for cloning...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the production...Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance." Antimicrobial...

... the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...infects over 250 million people worldwide. As we’ve previously seen in the CRISPR field, the lessons learned...

...environment. We’ve covered five model organisms in a previous blog post, but here are five more model organisms...conserved across species, studying the axolotl may reveal the potential humans have for a similar degree ... the sequencing of the entire axolotl genome, revealing an abundance of genes related to limb regeneration...Model for Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159...

... But conflict can also be good. In science, a reviewer’s critical comments on your manuscript is a form.... As you perform the experiments to address a reviewer’s concerns, you are making your hypothesis stronger...majority of conflicts I address in my current role revolve around missed tasks, stalled projects, and disagreements...particular teams at that given time. I think about how previous iterations of those teams resolved similar issues...

...their publications or as a review on a company website. It is important to review the data and protocols ...Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem overwhelming, especially...available resources and give yourself time to carefully review your options. If possible, try to select a couple...

...have higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1)....protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of...have lower background and higher contrast than previously developed NIR split probes (Tchekanda et al.,...near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021).   Table 1: Properties...

...sugar-containing molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology... have visible colonies growing on the membrane wherever an E. coli colony expressing a functional binding...  References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources...Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David...

...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...silent, they could identify the target genes of previously uncharacterized enhancers. The popular TALEN-...CRISPR activators and repressors are thought to be reversible once the effector is inactivated/removed from...KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced methylation...

... activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source....  Finding the right information When using a previously published system in your own work, you’ll need...

...protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More...Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their bulk (~27...which lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the...

...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...(also known as AAVrg) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...variables, so be sure to check the literature for previous work in your experimental system. Table 2: AAV...

...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...or not. Choose wisely This point ties into the previous one too. I would recommend applying for multiple...factors that can make or break a lab experience. Previous Addgene blogs have discussed the process that ... but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science...

... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...suitable for targeting FRET acceptors to proteins (reviewed in [Yan & Bruchez, 2015]). To get you started,...HTRF: a technology tailored for drug discovery–a review of theoretical aspects and recent applications....energy transfer as a spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID...

...yeast, flies, and even plants! In this blog we will review common uses of MoClo and the kits Addgene offers...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American...

...of plasmid prep do you need? As we covered in a previous post on plasmid preps, not all plasmid preps are...purification kits make it easier than others to prevent quality issues. For example, some kits reduce the...weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore... high level of consistency between lots, then reviewing their quality assurance documentation can help...