We narrowed to 479 results for: Ret;
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TypeBlog PostPublishedOct. 15, 2024, 1:15 p.m....for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids. Please be aware that this ordering...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...
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Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...Disequilibrium, Disillusion and The Postdoc Dilemma” which pretty much said it all. She cited the oft quoted statistic... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....speechless (the club of people who have been in space is pretty small). Therefore, I was delighted to be invited...During the panel, an artist was sketching her interpretation of the discussion in markers on a whiteboard... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....protein-protein interactions through multicolor labeling and FRET. Specialty constructs for biosensing. Over 300... tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just such a great collection ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address ... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m.... Additional Resources Learn how you can use FRET to study protein interactions Learn about other ...CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein interactions ... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....graduate students, post-docs, and PIs. I won’t pretend that that there aren’t problems with the academic...academic enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed...developed a set of functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit... -
The Strength of Story Telling
TypeBlog PostPublishedDec. 19, 2017, 2 p.m....be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....not require any additional reagents or lab work (Pacouret, 2017). Moreover, the sequencing data from multiple...195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...In addition, chimeric antibodies will inevitably retain some sequence from their parental species which... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m.... XP and melanoma more broadly. “This [award] is pretty great for us,” Wang said. “We were planning on ...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m..... Over the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive... sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward with shipping... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ... -
Fluorescent Proteins 101: Luciferase
TypeBlog PostPublishedJune 24, 2014, 3:59 p.m....Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m.... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot. Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...