We narrowed to 501 results for: SUB

...around the lens. It allowed me to focus on the subject after I’ve left the room. Microphone: Lastly, I...shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just out of frame allowing...camera from a distance if I needed to be close on a subject, or I could manually control the camera by hand...

...a glycoprotein that consists of four identical subunits that can each bind one molecule of biotin. Biotin...surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting...to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith...

...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...that emits light upon reacting with a specific substrate. The emitted light, or chemiluminescence, is detected...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

...published and unpublished experiments on similar subjects in a single, searchable space. Finally there are...2018). In this ideal situation, a researcher would submit an experimental design to a journal prior to performing...platform for pre-registration wherein researchers can submit designs and transparently reveal them upon completing...

...these fragments can be cloned into E. coli and subsequent proteins screened for cell-binding abilities ...proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity.  First, cut...room temperature. After this, it is helpful to subject your membrane to several freeze/thaw cycles to ...

...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...based on molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

...Fluorescence refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic...enzyme variant that covalently reacts with a small substrate ligand motif attached to a fluorophore or other...on a protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications...

...is it? What parts of it move when you feed it substrate or add an essential cofactor? How many binding...especially since the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host ...Ermolenko, Dmitri N., et al. "Observation of intersubunit movement of the ribosome in solution using FRET...

...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

...eluting the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed...the plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over...

...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

...four! (Note: For cases where the same name was submitted by more than one person, we selected the finalist...finalist based on the first time of submission.)     We love the way the names Aavery, Addy, and Cappy...

...over, we will host other contests in the future. Subscribe to the blog to stay in the know!     No pranks...see a reference to this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find...

... last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re excited to... 17, 2019   Lab material tree We often get submissions of decorations created from lab materials. This...

...fantastic team of high school students who recently submitted to the journal with funding from Addgene. Listen...personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target audience...

...entry will reveal additional data including the subject’s age at the time of injection and how long the ...contributed data so far and we encourage you to submit data from your experiments. You will be acknowledged...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...

...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

... Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick their ...Nika Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up...