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We narrowed to 490 results for: SUB

Showing: 141 - 160 of 490 results

  1. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...wet transfer system the sandwich is completely submerged in a tank filled with a transfer buffer formulated...that emits light upon reacting with a specific substrate. The emitted light, or chemiluminescence, is detected...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...these fragments can be cloned into E. coli and subsequent proteins screened for cell-binding abilities ...proteins directly onto the nitrocellulose for subsequent probing for cell-binding activity.  First, cut...room temperature. After this, it is helpful to subject your membrane to several freeze/thaw cycles to ...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Fluorescence refers to the emission of light by a substance (fluorophore) that has absorbed light or electromagnetic...variant tag that covalently reacts with a small substrate ligand motif attached to a fluorophore. When paired...on a protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...based on molecular weight by SDS-PAGE, and are subsequently transferred to a membrane. A primary antibody...of antibodies is used to both identify the cell subsets of interest from among the mixture and to measure...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...is it? What parts of it move when you feed it substrate or add an essential cofactor? How many binding...especially since the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host ...Ermolenko, Dmitri N., et al. "Observation of intersubunit movement of the ribosome in solution using FRET...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

  7. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...eluting the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed...the plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over...

  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

  9. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...four! (Note: For cases where the same name was submitted by more than one person, we selected the finalist...finalist based on the first time of submission.)     We love the way the names Aavery, Addy, and Cappy...

  10. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ...over, we will host other contests in the future. Subscribe to the blog to stay in the know!     No pranks...see a reference to this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find...

  11. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ... last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re excited to... 17, 2019   Lab material tree We often get submissions of decorations created from lab materials. This...

  14. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...entry will reveal additional data including the subject’s age at the time of injection and how long the ...contributed data so far and we encourage you to submit data from your experiments. You will be acknowledged...

  15. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...

  16. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

  17. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ... Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick their ...Nika Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up...

  18. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...hypothesis was that trained muscle would produce a substance with beneficial effects on the brain,” Ruas said...produces an enzyme that purges the body of harmful substances. So in this context the muscle's function is ...

  20. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...
Showing: 141 - 160 of 490 results