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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 378 results for: SUP

Showing: 141 - 160 of 378 results

  1. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...demands of advanced microscopy applications such as super resolution, electron, and expansion microscopy. ...with similar capabilities. If you’re looking for a super stable FP for your next microscopy experiment, consider...

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization...

  3. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...spontaneously released from the producer cells into the supernatant during the production phase. However, this has...centrifugation steps to separate cell culture supernatant containing exo-AVVs from producer cells, and ...

  4. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...ultimately responsible for outcomes. Be ready to support your team and take responsibility if things go ...want to know about right away? Provide adequate support. The manager must be available to answer questions...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...an even newer green fluorescent protein that is supposed to be extremely bright. In S. cerevisiae, we've...

  7. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...go about starting a lab? Obviously, funding and support are crucial parameters, and familiarity with equipment...seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time!” George...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...genetic electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared...yeast display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and ...protein expression Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck...

  9. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...between layers are muddled due to the passage of supernatant through each layer. The right panel illustrates...before and after ultracentrifugation. The viral supernatant migrates through increasingly dense layers until...

  10. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group meetings and...the BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist ...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...education and policy and to draw attention and support to work in their field. It can be hugely beneficial...ComSciCon, our primary mission is to strengthen and support the network of graduate students who are passionate...

  13. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

  14. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...also improve localization accuracy when used in super-resolution microscopy by reducing the distance between...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild...proceeds from the maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...measuring the amount of radioactivity in the culture supernatant (this is indicative of protein degradation). ...variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). They are connected by a...

  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow...

  18. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...know about the vendor and the vendor’s technical support? An antibody from a vendor that you use frequently... with a responsive and knowledgeable technical support team, is a safer bet than one from a large distributor...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...lab. Granted, I currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular...problem solving skills’? As a part of the Scientific Support team, much of my day is spent helping to resolve...
Showing: 141 - 160 of 378 results