We narrowed to 635 results for: UTY;

...Promega’s Advanced Technologies group as well as evolution of the coelenterzine substrate to the more stable...making it non-optimal for in vivo imaging. The solution is to couple Nluc with a red-shifted fluor. Here...other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase Many intracellular sensors... from Addgene and we're excited to see how you utilize them in your group! Many thanks to our guest ...Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem ...

... the University of Manitoba. Our lab has been utilizing chemogenetics (Designer Receptors Exclusively ...fact that AAVs themselves are not pathogenic, precautions should still be taken when using them. There ...resistant to alcohol-based disinfectants. A 10% bleach solution should be used to clean AAVs from workspaces. ...being made to make them even more useful for therapeutic purposes. The University of Pennsylvania and ...Cas9 but have the potential to deliver other therapeutic molecules such as microRNAs (miRNAs), short hairpin...

...in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of the cell – never ...expensive, and not to mention frustrating. One elegant solution to sidestep the antibody problem is to tag the...tagging. Using our 96-format pipeline, we can routinely achieve 5-30% tag knock-in efficiency across different...experiments using the csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow...should be pursued, however, clonal lines must be cautiously analyzed for any damage (indels) to the promoter...

...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from...from the Mirabilis mosaic virus, the constitutively active M24 promoter has been modified with duplicated...large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is functional...antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it can be used to select both...

...Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off simply ...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects...

...legal approvals so the plasmids are ready for distribution as soon as the paper is published, while giving...with permission to release their plasmids for distribution, and we’ve found that this step was sometimes...already moved on to their next position. We do routinely review deposits that are left in the hold status... published we’ll get the plasmids online for distribution instead of risking leaving them on hold indefinitely...

... shy away from looking beyond its confines. The beauty of serendipity often emerges at the intersections... key to unlocking new perspectives or even revolutionizing your research. Why serendipity matters Serendipity...lead to unexpected discoveries and innovative solutions. Allowing for serendipity in your lab research...benefits by encouraging activities beyond the lab routine. It encourages you to think beyond the confines...PhDs in their careers with actionable steps and execution plans.    More resources on the Addgene blog ...

... a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager is ...organization and feel the importance of their contributions. Flexible work schedule Flexibility to manage...personal growth do not exist or if they feel underutilized in their present positions By removing the...will address more specific tactics managers can utilize to be in touch with the needs of the team and to...

...to complete your story: an introduction, and a resolution.   Fig. 2: The structure of a story - ... left out, which can be a big challenge!  The resolution Throughout your conflict, you’re encouraging ...audience - via the scientific method - to the resolution, or the ending of the story.  At this point you...complex story, and then build on that in your resolution to really dig into the significance of the results...

...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...of how to appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable...risk perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental...unsettled science. Understanding just how the public utilizes insights gleaned from scientific research is, ...

...structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon...Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP... to FRET and stochastic single-molecule super resolution imaging. mNeonGreen also has little sequence ...Addgene! References Ai, Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable version...

...deftly coordinated by the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

...a single person. With thousands of scientists routinely using and thinking about FPs, it would be great... designed with the goal of encouraging user contributions (using simple web forms) while also maintaining...and we very much appreciate even those small contributions). Of course, no community-editable database ... include incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum...

...did To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and ...room temperature and used the color of the liquid solution and pH paper to measure the pH. We compared the...note that the change in pH only occurred when the solution thawed, as the CO2 was confined to the tube headspace...et al., 2013). However, the pH changes in our solutions are transient and return to normal once the tube...

...treatment options, as well as non-pharmacological solutions to modify the course of this condition are urgently...glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva, Universidad ...related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology resources...Biology Resources Learn about Global Plasmid Distribution ...

...commonly used ones for transfers between academic institutions.  What is the UBMTA, you ask? Well, organizations... agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which ... researchers additionally have the option of distributing under Addgene’s Industry MTA (IMTA), which allows...depositing institute that decides the terms of distribution for their plasmids. MTAs at Addgene Since Addgene...

...(accession numbers, reagent details, author contributions, etc.) before their paper can be published. ...do. All of that helps, but it’s only a partial solution.  How do we get where we want to be? The part ... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...across life and physical science, and editorial contribution to the strategic development of Cell Press. ...

...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...

... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic.... At a very low volumetric density and with a resolution of less than 100 µm, the ARG reporters could ...group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes...

...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...plan manage risk. My job and my team’s job is to routinely assess the efficiency and effectiveness of the...auditor (CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute...