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We narrowed to 484 results for: Vit

Showing: 141 - 160 of 484 results

  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...relationship with authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always...make the relationship stronger.  If appropriate, invite them to speak for your organization, mentoring ... simple question so I accepted the connection invitation. They now message me frequently and ask me how...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... on bacterial expression systems here) and the activity of the plant compounds on these proteins will ... be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar Tsakanova...image cortical neurons and study changes in neural activity after ischemic brain injury. Additionally, optogenetic...

  3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA... if we could leverage this model (built from in vitro studies) to boost the efficiency of sequence replacement...enables sequence replacement without cutting Our in vitro results also demonstrated that catalytically inactive...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...from the 3’ end, while its native endonuclease activity degrades the displaced strand.   Figure... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that activate different...neuroglia, and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD ...chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.   ...

  6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...the insert. Gateway® technology (developed by InvitrogenTM) is essentially a more modern version of this...CcdB-resistant strain is ccdB Survival™ from InvitrogenTM. Using either DB3.1 or ccdB SurvivalTM, one ...a variety of useful resources and protocols! We invite feedback from all members of the research community...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...in cells, and without something to block RNase activity upon cell lysis, RNA is degraded. To effectively...-based solutions that work by inhibiting RNase activity within cells or tissues- they don’t actually chemically...A (1974) The effect of ammonium sulfate on the activity of ribonuclease A. Biochimica et Biophysica Acta...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...ability to post a preprint as evidence of their productivity that can be shared with hiring and review committees...encourage and consider preprints as demonstration of productivity.  Part of an Open Scientific Communication Ecosystem...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... you to share and build support for your ideas, invite others into decision making processes, and even...usually) presenting either to share knowledge or to invite critique onto an ongoing project. So whenever you...Conflict Resolution Transferable Skills Guide: Creativity...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...sensors for a diverse set of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are...progesterone, androgens, and estrogens), fat-soluble vitamins A and D, thyroid hormone, fatty acids, oxysterols...transfection platform for assaying transcriptional activity, for example, was a key development in the functional...

  11. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...personal identity. We do this through a variety of activities, including creating educational materials and...addition to events that we put on, we are often invited to present our materials or speak about our experiences...our science communication posts Extracurricular activities for a strong science career path Resources on...

  12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2 gene. Videira’s group... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...phenotypes. Developing a rapid test for the functional activity of VUSs will enable more accurate diagnosis of...

  13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...interact.  When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...BirA* enzyme. When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...proteins Y and Z part ways, BirA* biotinylation activity ceases. After biotin tagging of proteins in both...

  15. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ...more stringent, and it avoided any days of lost productivity waiting for follow-up DNA oligos to arrive. ...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

  16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...-base sequence. Double mismatches ablated Cpf1 activity, except when they were present in the 3’ end of... as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target...found that both orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... (500 to 580 nm) over the course of 18 hours in vitro (see figure 1B in Terskikh et al.) and even displays...showed that their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus...changes in oxygen, temperature, and pH, but timer activity can be calibrated to these different conditions...

  18. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events...

  19. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...Intraperitoneal (IP) injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen...organs. Restraint: scruffing Location: peritoneal cavity Gauge: 25–27 Volume: < 2–3 ml Skill required: intermediate...tail vein) Intraperitoneal Scruffing Peritoneal cavity 25–27 < 2–3 mL Intermediate Intramuscular Mechanical...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...Plasmids containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 ...ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA...nor are these methods limited exclusively to in vitro cell culture. The user should review the current...
Showing: 141 - 160 of 484 results