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We narrowed to 144 results for: aav vector plasmid

Showing: 141 - 144 of 144 results
  1. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...Zhang showed they could package LITEs in viral vectors and genetically target them to specific cell populations...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So...
  2. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ... Bioengineering Find the optogenetic repressor plasmids Cytosine base editors with minimized off-target...paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter crescentus Figure...once. Read the paper in mBio Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system...lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression of ... Read the paper in Scientific Reports Find the plasmids Enhanced CRISPR-based DNA demethylation ...
  3. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...
  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... NGS Plasmid Validation: SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality...days, we would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while...while more recently we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation...lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing data collection...transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC team to...With our outline of individual steps to automate plasmid sequence assembly, and possible solutions for each...up with our sequencing instrument (hundreds of plasmids each week!), we already planned to use cloud computing...
Showing: 141 - 144 of 144 results