...Schwiesow A leading cause of death worldwide, cardiovascular disease is driven by atherogenic lipoproteins...been shown to have a strong correlation with cardiovascular disease and indirect measurement of ApoB-LPs...concentration, Steven Farber and colleagues at the Carnegie Institute for Science have developed the LipoGlo...to aid in the understanding and treatment of cardiovascular disease. Find the LipoGlo plasmids at Addgene...

... of researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent...at scientific conferences. In my nearly 20-year career in science, I have never attended a scientific ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent...alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint effort of...

... a lab that will be a good fit for you and your career ambitions. First, how does one stand out amongst...interview process. What if I want a non-academic career path?  If you are considering this, make sure to...will want in year 6. Have lab alumni taken diverse career paths? Ask other lab members how the advisor treats...inquiries. If you know you would like to head for a career in pharma or biotech, this is one case where the...

...application to assess your eligibility, interest, and career fit. Most internships share a common set of criteria...potential, and drive. Read the associated prompt carefully, plan your response, and let your inner wordsmith...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ... Check out other tools and resources for early career researchers Applying for jobs? Check out these ...

...materials to specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are...infection) and delivering a genetic payload. This cargo is typically their own genome, but this step can...cell) can be tuned so that the copies of library cargo delivered are controllable. For many screening applications...essential to deliver a single copy of your library cargo to isolate the effects of each variable being manipulated...

...Regenerative Biology is to understand the basis for cardiovascular and metabolic human diseases. They do that ...efficiently generate human stem cells edited to carry mutant versions of 15 different disease-associated... target a large number of genes involved in cardiovascular and metabolic diseases. We are knocking out... type relevant to the disease in question – cardiomyocytes, adipocytes or neurons, for instance – to see...

...Although it goes without saying that one should take care whenever doing any type of DNA or RNA purification...purification to avoid contamination, take extra care when performing RNA extraction. RNA is inherently not as ...problem with no-kit RNA extraction methods is the carryover of DNA that can potentially complicate results...extracting, to only take the aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another...

...that’s an important soft skill to carry into the next phase of your career.  Happy defining! Additional...other transferable skills Find all of our science career related blog posts Read our science communication...communication blog posts Resources on Addgene.org Find careers at Addgene ...

... the Institutions” promotes gender equality in careers through structural change in research organizations...hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast cancers, and ...fractures. The overall economic savings to the US healthcare system was $35.2 billion (or $140 per $1 invested...Extracurricular Activities for a Strong Science Career Path Additional Resources Check Out Vidoes of...

... about a Variety of Science Careers Resources on Addgene.org Find Career Opportunities at Addgene   ... - securing higher salaries. This decision will carry over into other aspects of postdocs’ lives. We know...plans and budgets working around issues such as childcare using money from their increased salaries. In ...

... on various applications throughout my graduate career, and helped receive funding towards my PhD project...Empowers the writer, And offers insight into a career as a Professor. Step 1: Your PI forwards you...criteria, and award administration information. Carefully read over all parts until all aspects are understood...Grant Application" by Vid Mohan-Ram in Science Careers "Beginnings - How to write your first grant proposal...

...non-academic science careers Resources on Addgene.org: Sign up to receive Addgene’s Science Career Guide Check...rather than underdressed, but read emails from HR carefully because sometimes companies have relaxed dress...conferencing instructions you received from HR carefully. And even if you don’t have technical challenges...

...2010 Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...

... continue to shape the sector throughout their careers (McDowell et al, F1000Research, 2014; Dolan, F100Research...established, as they embark on or continue their careers in science-related fields. In particular, two recent... students in areas such as teaching salaries, healthcare access, and benefits; however, this may come ...Affairs at ASBMB Adam Fagen, PhD - Director, Early Career Scientist Segment at AAAS Marnie Gelbart, PhD -...

... deletion strains with overexpression plasmids carrying MET/Met genes from either S. pombe or S. cerevisiae...uracil, but contain methionine. (This allows cells carrying nonfunctional as well as functional MET genes ...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...Basic Lab Techniques High School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast...

...However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been challenging...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...It is an icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome...single dose of subretinal administration of rAAV2 carrying the human gene RPE65, patients with clinical diagnosis...

..., to congenital disorders. A series of studies carried out on an isolated population in Sweden found that...that decreased their likelihood of death from cardiovascular disease but increased their risk of death from...paternal grandmothers early food supply influenced cardiovascular mortality of the female grandchildren." BMC...Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular and diabetes mortality determined by nutrition...

...www.FinishYourThesis.com and is one of many posts in our career series.  “The only healthy communication style ...into learning opportunities as you are advancing career, you will become the independent, assertive, and...learn more about assertiveness skills is Dale Carnegie’s book “How To Win Friends and Influence People...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post ...

...their 30s who have quit their academic science careers in order to head to Ireland and take a chance as...product. It was a small project, not a full-blown career, but it was enough to start us down the entrepreneurial...started things up for real: bank account, business cards, incorporation, laboratory lease, etc. I still flip...allowed us to really analyze what we want out of our careers: to build something new and beautiful, to engage...

... homologous recombination events: The plasmid carrying the desired genetic modification integrates into...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for... New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes. Applied...