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We narrowed to 256 results for: human dat

Showing: 141 - 160 of 256 results

  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...used in human clinical trials. An off-target rate of 5% is far too high when considering human patients...likely will not have a significant impact on your data. You are trying to generate a knockout cell line...there are gRNA softwares available which score candidate gRNAs for on-target and off-target event efficiencies...them will also give you a specific list of the candidate off-target sites. For example, if there are 10... review of other targeted sequencing methods. Candidate site sequencing Sites with high sequence similarity...DNA binding sites within your experiment. For a candidate site-specific approach, mRNA quantification or...CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit          ...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...implemented by applying the Golden Gate cloning method to human proteins known as Pumilio/fem-3 mRNA binding factors...library even better, we would be very happy to share updated versions of the kit.” Reference Abil Z, Denard...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...show the gRNAs that were used for activation in a human system, search for ‘sapiens activate’ to filter ...many validated gRNAs in our newly curated Validated gRNA Target Sequence Table. What are validated gRNAs...searchable and sortable datatable that contains validated gRNA sequences. Validated in this context refers...effects are specific to that locus. The data in the validated gRNA table has been derived from scientists...containing the validated gRNA sequences alone. Sequence submission is welcomed for validated gRNAs that are...sequences here. Features of the validated gRNA table You can access the datatable here. Current table columns... exclude the data that you are not interested in. You can use the carats in the datatable headers to sort...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing...Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids Research...approaches, and provide updates on how best to design gRNAs. You can also find validated gRNAs for your next...next experiment in Addgene's Validated gRNA Sequence Datatable. A more extended discussion of these subjects...Different databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...rise to an off-target effect, there’s not enough data to fully predict which sites will and will not show...

  5. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...world to conduct robust investigations into the human transcriptome. The MORF library is only one of 22...launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll continue growing...fourth edition of our Plasmids 101 eBook. This updated version of one of our most beloved resources has...to make it more accessible. Other accessibility updates include a printer-friendly design and alt text ...on all images. We have also made accessibility updates to our website, which receives 3.8 million visitors...

  6. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...research tools is particularly interesting. Although humans have known about bioluminescent organisms and their...for some time – there are records describing them dating back to the first century A.D. – the first time...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...thermophilus which have been used for gene editing in human cells without cross-talk in cognate sgRNA binding...understanding the spatio-temporal regulation of human genes. The plasmids are all available at Addgene...These three enzymes were chosen because they cut human and mouse coding regions infrequently; any MluI,...understanding the role of long noncoding RNAs (lncRNAs) in human health and disease. A primary question in their ...He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison of different... in E. coli. The authors used pXPG to study the human GM-CSF promoter and enhancer, a finely regulated...BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin, which releases energy...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...research focuses on its potential applications for human medicine (Waltz, 2016), the potential of CRISPR ...alternative genome editing technologies especially in human cells. In contrast to homology directed repair, ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your gRNA Additional ...Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for Your Next Experiment ...

  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...to Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome...#12DaysofCRISPR and blog updates We hope you enjoyed the #12DaysofCRISPR, a celebration of our updated blog resources...Editing with CRISPR-Cpf1 Day 4: Edit validation: CRISPR 101: Validating Your Genomic Edit and Sequencing ... you.) With these goals in mind, we set out to update our web resources and plasmid pages to make them...them more useful and easy to navigate. We also updated some our most popular CRISPR blogs to keep them ...modification. In addition to these subpages, our updated CRISPR Guide and CRISPR History provide a great...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...enabled brings us closer to making an impact on humanity's greatest challenges. Thank you for being part... characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers...extensive plasmid database — because we believe that innovation flourishes when data are shared. The scientific...describing viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... mutagenesis induced by CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9 (2013): 822-826... profiling of CRISPR-Cas9 off-target effects in human cells." Nature methods 12.3 (2015): 237-243. PubMed...standardized validation techniques for academia, industry, and especially the clinic. Popular validation assays...guest blogger Søren Hough, a Biochemistry PhD candidate at the University of Cambridge. One of the most...important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR genome editing...mutation and not an edit in the target gene. The validation process, also known as CRISPR genotyping, is ... are insufficient As discussed in CRISPR 101: Validating Your Genome Edit, there are a variety of options...

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ... exact person monitoring submissions (usually a Human Resources representative). Pro tip: if there is ...an appropriate liaison, but you can use alumni databases, friends, and even relatives to network your way...

  13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...approach, ranging from ~10% expression in primary human T cells and hematopoietic stem and progenitor cells...Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089...solution is to include an in-frame degron, or degradation sequence, upstream of the 5’ splice donor and...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...mouse and large animal models with mutations in human-relevant exons 45-55, rather than in the traditional.... Gene-editing summit supports some research in human embryos. Nature. 2015 Dec 3. doi:10.1038/nature.2015.18947...Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...: A database of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository...molecular biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...Knowledge Base: A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies: ...Reagent and Data Repositories These links can help you find experimental and/or validation data on molecular... Flame plasmid pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze sequence...sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled from publications...nucleotide or protein sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence...

  16. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...performed to take the drug to the pre-clinical and human trial phases.   So how does CRISPR factor into this workflow...be most effective. Once drug candidates are isolated, they can be validated for specificity to their target... be identified if it isn’t known. If a strong candidate emerges, downstream optimization is performed ...already exist a drug which targets some of the candidate genes! Disease model generation CRISPR has made...effectiveness prior to patient treatment. Target validation When a target is known and a drug is developed... to measure the specificity of that target and validate the hit compound. The standard is to test the ...BRCA-deficient breast cancer and identified several candidate inhibitors of gene X in a screen. You generate...

  18. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...on hold for an hour. When you finally speak to a human being, you may not even get a solution to the problem... with all of our resources, we will continually update the Help Center to make sure that, whatever your...

  19. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...or 130 ms on and 0.7 or 2.5 s off, for mouse or human receptors, respectively). Using GRABDA sensors in...freely behaving mice to gather the following types of data: Report time-course and concentration of released...freely moving mice to gather the following types of data: Report endogenous DA release from a single dopaminergic...Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity to the...

  20. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ..."Methods for monitoring autophagy from yeast to human." Autophagy 3.3 (2007): 181-206. PubMed PMID: 17224625...including organelles, are targeted to lysosomes for degradation. Autophagy is a dynamic process which involves...materials to be degraded to the lysosome, and degradation of autophagic substrates inside the lysosome....autophagasomal membranes, which leads to its degradation by autophagy. The RFP-LC3∆G, however, can not...
Showing: 141 - 160 of 256 results