...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

...published, a bioRxiv preprint that questions the conclusion of inter-homologue recombination was released...off-target effects attributable to CRISPR editing. Conclusions & implications One striking feature of this paper...editing rates vary widely from gRNA to gRNA, it’s unclear where this study would fall in terms of editing...

... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...

.... In the case of stem cells, these factors may include cell type, the disease being treated, and other...immunological, and environmental factors. How to include sex in experimental design Recognizing the importance...Institutes of Health (NIH) now requires that sex be included as a biological variable in all NIH-funded research...

...fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine steroids... to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...interface, expanded numbers of curated molecules, including 400 or so coregulators, and a greatly expanded...

...paragraph and point out words or sentences that are unclear. We’re so used to conversing with other scientists...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

...nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as CAR-T cells...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but ...recombinant antibodies as well? Our collection includes a mouse and a chimeric rabbit (original mouse ...

... recognized by distinct proteins and result in unclear transcriptional consequences (Sprujit 2013). Enzymatic...local gene expression, confounding any potential conclusions about the role of methylation (Hrit et al. 2018... many effects independent of dCas9 targeting, including modifying other sites in the genome and directly...

...the quality of other services that we provide, including viral preps and pooled libraries. As anyone who...throughout the library preparation process and includes Picogreen quantification of various intermediate...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter...

... of infection and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré...seven institutions involved in the effort. This inclusive and open approach by all involved definitely contributed...transformed the assay’s capabilities. Specifically this includes primer directed isothermal RNA amplification, ...

... transferable into multiple bacterial species including several involved in human disease. However, the...every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer...Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic strains and environmental isolates....

...series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and ...detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials (...triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

... mice. While not an exhaustive list, these can include what’s being injected, the type of experiment, ...method. Many different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses.... the name suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs...

...NgAgo optimization before we can make any major conclusions: That said, many people are having difficulty...Some great places to get your results out there include: Google Groups - I've specifically set up a forum...Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain cells...

...can wrap the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) ...assays used in the papers describing the FPs above including molecular sizing columns and native gels fail ... Anatomy and Structural Biology. His interests include the quality control of secretory proteins in the...

...current genome engineering applications of CRISPR including: generating single or multi-gene knockouts in ...) that harbors two surface exposed cysteines, including the native C547, allowing for ligation to pyridyl...Senior Scientist at Addgene.  Andrew's interests include genome engineering and microbiology.     References...

... different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our model infers...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...

... Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look at this new... deposit prior to publication and were able to include specific plasmid ID numbers. If you know the ID...suggest direct links to other types of searches including depositing labs and institutions. For example,...

...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...