...permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol. Although the...targets, we usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions....1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal perm/fix solution depended...

...human endeavor including but not limited to biotechnology, chemistry, architecture, engineering, mathematics... sector are transferrable to industry such as technology transfer, communications, medical writing, compliance...Casseus Law. Driven by her own love of science and technology, Rachel is proud to assist companies and foreign...

...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...without staining; simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose...

... focuses on producing biotechnologies in the areas of genome editing, immunology, and gene therapy.   ...

...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...

...podcast that introduces you to 4 new plasmid technologies from one of our recent newsletters. Listen ...you enjoy this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids...

...views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science Communications ...the end of the post also really helped put this technology into a concrete context. I recommend this post...

... for more detailed information about this new technology. Our Plasmids 101 series continues to grab scientists...questions or if you have a topic, researcher, or technology you would like us to feature in the coming year...

...Sample 6 16:27 - 18:02 : Benefits of the Sample 6 technology and how It works 18:02 - 19:56 : Why Sample 6... could succeed in the market or to focus on a technology that one is passionate about developing? 38:00...

... and adopted by the Association of University Technology Managers (AUTM). This MTA provides many benefits...and the receiving institution. Therefore, many technology transfer officers are familiar with the UBMTA...

...perturbations – in the case of screens using CRISPR technology, gRNAs to knockout genes – are essential screening... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...

... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ...

...knockout of genes in the immune system. Nature Immunology, 25(1), Article 1. https://doi.org/10.1038/s41590...subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347. https://doi.org/10.1038/s41590...

... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this...

...reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared...

...intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such...

...rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. PubMed... modify clinical-grade T cells to target CD19. Immunol Rev. 2014 Jan;257(1):181-90. doi: 10.1111/imr.12137...

...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much...