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We narrowed to 440 results for: pes;

Showing: 141 - 160 of 440 results

  1. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...expression at certain loci to create changes in phenotypes. For example, CRISPR activation has been used...and Gersbach, 2015; Zetsche et al., 2015). These types of CRISPR activation are also reversible: once the...et al., 2016). To learn more about the different types of Cas9 activators, check out our blog post covering...

  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...There are many, many different types of experiments carried out by scientists every day. Although the...plasmids would address and answer these questions. Types of control plasmids Part of planning your experiment...expressing the independent variable. There are two types of replicate controls: technical and biological....

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...require interaction with the data authors. In some types of research, raw data requires huge amounts of storage...calling it a “sisyphean task” given the endless types of data). Rather, PLOS appears to leave the scope...provide data in formats exported to common file types? And how long should data be archived? Simplifying...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Because transcription machinery differs between cell types or organisms, promoters must be similarly variable...derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc) require unique promoters...an enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...to admit that paper-based record keeping barely copes with the growing amounts of digital data generated...functionalities with experiment records. Many different types of ELNs are available on the market today, ranging...showed that It is important that an ELN supports all types of data, which enables researchers to store everything...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...specific 3-dimensional epitopes. Different sample processing steps can alter epitopes in different ways, ...my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based on the...

  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...bind to epitopes of interest that are found in the extracellular space. The targeted epitopes can be motifs...simply need to label the lineage markers of all cell types except CD8+ T cells. We might choose the markers...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...lymphocyte population from a tumor sample. Many cell types can be found in the tumor microenvironment (tumor...Around 34% of the cells are neither of the two cell types (likely B cells and others). Almost no cells stain...in this blog post. First, you learned about the types of scattering that occur when a cell is pulsed by...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...DNA viruses, such as adeno-associated virus, herpesviruses, hepatitis B virus, adenoviruses, and human ...linear proviral DNA is poised for integration, two types of circular episomes can be generated through either...This makes IDLVs an attractive choice for certain types of clinical or basic research applications. Considerations...

  10. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...target site sequence at the site of insertion.  Types of transposons Transposable elements are split into...the size of their host’s genome.   There are two types of Class 1 TEs: those with long terminal repeats... used in the lab While there are many different types of transposons, DNA transposons are most commonly...

  11. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ... knock-in efficiency across different stem cell types. This has facilitated easy generation of clonal ...choice for epitope tagging in diverse stem cell types. Hundreds of genes can be epitope-tagged in a couple...large protein cohorts in diverse mammalian cell types. A detailed step by step protocol for the csRNP ...

  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...is induced by various types of interactions with their environment. These types of interactions to a large...growth properties, migration abilities, or other phenotypes that can be efficiently measured at the single...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    .... There are numerous variants for the different types of opsins available and their differences and uses...See the Deisseroth lab homepage for details about types of constructs available, instructions for obtaining...cell-type specific opsin expression. However, the types of opsins you are able to use are obviously limited...

  14. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...input in order to generate results. While different types of input are more user-friendly than others, this...less concern than data output as different input types can be readily converted.  Examples: “organism”,...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...and mumps. Sendai transduces a wide range of cell types and replicates in the cytoplasm independent of the...and protein delivery are better suited for these types of studies. What cells are reprogrammed will also...also affect the outcome, since not all cell types are easily attainable, or easy to reprogram. Basically...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...genome for genes that regulate a wide variety of phenotypes, including but not limited to cell growth and...chosen to represent the primary splice variant in 38 types of human cancer. Many of the transcripts are not...create protein expression constructs fused with epitopes or fluorophores, generate expression vectors with...investigating hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID:...

  17. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... proteins coat the virus and help determine the types of cells that it can infect. The VSV-G envelope ...it enables lentiviruses to infect many different types of cells. This means researchers who use VSV-G can...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... be packaged in your choice of five different serotypes.   We are so excited for all the ways this much-requested...Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg...

  20. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... she is currently working on a version that she hopes will change the intracellular localization of RNA...Her lab is currently investigating the issue and hopes to have a better understanding of the phenomenon...
Showing: 141 - 160 of 440 results