...quickly identify possible gene function. In this post, I will describe a useful and cost-effective method that...Thermo works well) with some added lysozyme, DNAse I, and protease inhibitor cocktail. Line the bottom ...Jessica C. Sacher, and Christine M. Szymanski. "Development of an Assay for the Identification of Receptor...

...Interest Waiver (NIW) allows for self-sponsorship of the I-140 application meaning that the category does not...and should help facilitate the interview process. I often suggest to international candidates that they...writing, clinical, regulatory, product development, business development, consulting, policy, big data and ...presented may not reflect the most current legal developments.  Further, this post may contain technical inaccuracies...necessary and often overlooked part of career development for foreign national researchers and entrepreneurs... Careers is a life science search and career development firm focused on connecting innovative, entrepreneurial...project management, alliance management, business development, finance, and operations. To learn more, visit...

...pnas.1915974116 Guerin K, Rego M, Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman... Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation...classes of neurons transiently express TH during development or have low levels of TH mRNA without expressing...

... Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition into...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

... to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms, both open-access....), The Nonhuman Primate in Nonclinical Drug Development and Safety Assessment (pp. 519–542). Academic....2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic antibodies for the treatment of...

...researchers to use this tool in their own work. She says, “I spend a lot of time packing, shipping, and sharing...at a variety of questions surrounding cancer development, metastasis, and drug resistance, Bhang says ...appropriate, non-overlapping targets for drug development - such that initial resistant populations can...tumor heterogeneity to drug discovery and the development of combination therapies.         References ...

...encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start using.... Merkx recently applied this “trick” to the development of additional color variants. This resulted in...

...What is the goal, who is my audience, and what do I want to be different for them when I’m done? What ...online resources. On the backend, our product and development teams have worked hard in increasing and improving... 2023, we hosted our very first professional development workshop, “Make a Greater Impact Using Clear ...

...just six weeks later. The Zika publication timeline I think we can speak for all the authors on the paper...and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré syndrome...health emergency and called for the fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes...timescale. Our article ultimately reported the development of a rapid design and prototyping pipeline that...necessary to advance the technology through product development, the regulatory process, and manufacturing so...

...thiol-containing acceptors in competition assays. “I believe that improved biosensors or biosensors that... Tsien who have played enormous roles in the development and dissemination of fluorescent protein tools...populations in the brain that contribute to the development of behavioral signs of opioid withdrawal before...

...can later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...Blocked by overlapping methylation Not affected PmeI Not affected Not affected Blocked by overlapping...

... follows are just a few suggestions based on what I have found to be especially beneficial during my own...scientists. Continuing professional development Professional development should be life long, and doesn’t...landing that next coveted job! Professional development (or lack thereof) One of the biggest challenges...programs lack any formal or mandatory professional development training. Further, even for those students and...take responsibility for your own professional development, not count on your institution, program, or adviser... a job fair, and utilizing your local career development center, if one exists. Get online! Review company... career development...

... Shah et al., “Do you know the sex of your cell?” I can’t tell you how many researchers I’ve talked to...harm humans or contribute to failures in drug development – costs far greater than those of doing the research...

...of chemically converting adenosine (A) to inosine (I, treated as G by the cell). Surprisingly, the team..., 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B....pathways that were considered problematic in the development of CBEs and ABEs. This post includes a lot of...

...pluripotent cells is embryonic stem cells (ESCs), which I discuss in detail below [2]. Multipotency: When a ...multiple cell types makes them useful models for developmental processes and promising therapeutic tools. The... and extraembryonic cell types. In mammalian development, cells found in the zygote through to the morula...

... as VSV-G, may be used across both systems. Below I provide a brief overview of a few popular gamma-retroviral... learn more about CRISPR and the most recent developments in the genome editing field. Lentiviral Plasmid...

...Addgene Viral Plasmids Which Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors...scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common forms of lab accidents...

...expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is strong...only in certain tissues or at certain times in development or there may be ways to turn them on or off at...tables above do not delve into the tissue or development-specific promoters available to scientists. Plasmids...

...aside, transcriptionally active dioxygenase  When I started to work on building a CRISPR/dCas9-based tool...TET1 to regulate TET1 activity and function in development. eLife 7:e34870. https://doi.org/10.7554/eLife...

..., Ishibashi, H., Yuasa, S., Takeda, S., Hasegawa, I., and Nakahara, K. (2011). Local and retrograde gene... J. M., Vandenberghe, L. H., Haute, C. V., Thiry, I., Deroose, C. M., Mortelmans, L., Wilson, J.M., Debyser...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...intracellular transport of AAV could facilitate the development of more powerful AAV serotypes, and perhaps lead...